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A kind of preparation method of three-dimensional chiral silver nanomaterial

A technology of silver nanoparticles and silver nanoparticles, which is applied in the direction of nanotechnology, can solve the problems of low assembly yield and uneven assembly structure, and achieve the goal of controlling growth size, avoiding instability and inhomogeneity, and improving specificity Effect

Active Publication Date: 2017-02-08
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problems of low assembly yield and inhomogeneous assembly structure in the chiral nanomaterials prepared by using chiral molecules in the prior art, and to provide a three-dimensional chiral silver nanomaterial with a DNA skeleton as a template. Preparation

Method used

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  • A kind of preparation method of three-dimensional chiral silver nanomaterial
  • A kind of preparation method of three-dimensional chiral silver nanomaterial
  • A kind of preparation method of three-dimensional chiral silver nanomaterial

Examples

Experimental program
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Effect test

Embodiment 1

[0047] (1) Preparation of DNA three-dimensional space skeleton

[0048] Four different single-strand DNAs were produced by Sangon Bioengineering (Shanghai) Co., Ltd.: the sequence of L1 is shown in SEQ ID NO.1; the sequence of L2 is shown in SEQ ID NO.2; the sequence of L3 is shown in SEQ ID NO.3 shown; the L4 sequence is shown in SEQ ID NO.4, and Table 1 shows the sequences of the four single-stranded DNAs used. The black italics are the base C sequences, and the positively charged Ag + Specifically adsorbed on the base C in the DNA backbone. The initial concentration of the 4 single-stranded DNAs is 100mM, respectively resuspended in 10mM tris-HCl buffer to a final concentration of 500nM, 4 kinds of DNA molecules, 50uL from each tube and mixed in a 1.5mL centrifuge tube, 95 ℃ water bath After 5 minutes, it was rapidly cooled to 4°C. During the cooling process, the four single-stranded DNAs hybridized with each other to form a DNA assembly with a three-dimensional spatial co...

Embodiment 2

[0056] (1) Preparation of DNA three-dimensional space skeleton

[0057] 4 different single-stranded DNAs, the L1 sequence is shown in SEQ ID NO.1; the L2 sequence is shown in SEQ ID NO.2; the L3 sequence is shown in SEQ ID NO.3; the L4 sequence is shown in SEQ ID NO.4 Table 1 shows the sequences of the four single-stranded DNAs used, the black italics are the base C sequences, and the positively charged Ag + Specifically adsorbed on the base C in the DNA backbone. The initial concentrations of the four single-stranded DNAs are all 100mM, and they are respectively resuspended in 10mMtris-HCl buffer to a final concentration of 100nM. For each of the four DNA molecules, 50uL equal volumes are mixed in a 1.5mL centrifuge tube, and placed in a 95°C water bath for 5 After a few minutes, it was naturally cooled to room temperature. During the cooling process, the four single-stranded DNAs hybridized with each other to form a space skeleton with DNA pyramids.

[0058] (2) DNA backbo...

Embodiment 3

[0068] Optical and structural characterization of prepared three-dimensional chiral silver nanoparticle assemblies

[0069] The product prepared in Example 1 was centrifuged at 15000r / min for 20 minutes, the supernatant was removed, resuspended in ultrapure water, and washed once with ultrapure water. Electron microscope characterization: 10 μL of the above-mentioned treated sample was dropped onto a copper grid supported by a carbon film, and dried under an infrared lamp. The electron microscope adopts JEOLJEM-2100 type electron microscope, and its accelerating voltage is 200kV, such as figure 1 and figure 2 as shown, figure 1 It is a high-magnification transmission electron microscope body with a helical structure of silver nanoparticles. It can be seen that the specific configuration of silver nanoparticles is that the four particles are not on the same horizontal plane, showing a clockwise helical structure; figure 2 It is a low-magnification transmission electron mic...

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Abstract

The invention belongs to the field of nanometer materials and optics, and provides a preparing method of a three-dimensional chiral silver nanometer material using DNA (deoxyribonucleic acid) frameworks as templates. According to the method, the DNA frameworks are used as templates for absorbing silver nanometer ions, and the silver nanometer ions are reduced into silver nanometer particles through reducing agents. The method concretely comprises the following steps that (1) DNA three-dimensional frameworks are prepared; (2) the DNA frameworks absorb the silver nanometer particles; (3) the silver nanometer ions which are not adsorbed are removed through ultrafiltration; (4) the silver nanometer particles are reduced through reducing agents. The problems of low assembling yield, nonuniform assembling body structure and the like during the preparation of chiral nanometer materials by using chiral molecules in the prior art are solved, and the preparing method of the three-dimensional chiral silver nanometer material using the DNA frameworks as the templates is provided. The method has the advantages that the growth dimension of the silver nanometer particles can be effectively controlled through regulating the concentration of the silver nanometer ions and the reducing agents in the system, and a transmission electron microscope result shows that the four silver nanometer particles are in spiral arrangement, so that the chiral signal resources are explained.

Description

technical field [0001] The invention belongs to the field of nanomaterials and optics, and relates to a preparation method of a three-dimensional chiral silver nanomaterial using a DNA skeleton as a template. Background technique [0002] Nano is a unit to measure the scale of materials, 1nm is equal to 10 -9 Meters, equivalent to the size of 10 atoms. Nanotechnology is an interdisciplinary research technology that accurately identifies, observes and controls the number, type and geometric configuration of substances at the single atomic or molecular scale. The emergence of nanotechnology has brought human beings into a new field - "mesoscopic". It exists between the microcosm and the macrocosm, and the space ranges from 1 to 100nm. The substances in this narrow world have many peculiar optical, magnetic, electrical and mechanical properties. materials are collectively referred to as nanomaterials. [0003] The preparation of chiral nanomaterials is a hot research direct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B82Y40/00
Inventor 严文静章建浩
Owner NANJING AGRICULTURAL UNIVERSITY
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