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Populus diversifolia sexless breeding method based on gene regulatory network

A gene regulation network and asexual breeding technology, applied in the field of breeding, can solve the problems of low cutting propagation, difficulty, poor rooting ability, etc., and achieve the effect of promoting the breeding of stress-resistant clones and improving the reproduction rate

Active Publication Date: 2015-02-18
BEIJING FORESTRY UNIVERSITY
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  • Description
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Problems solved by technology

However, the rooting ability of Populus euphratica is relatively poor. When cutting, it is very difficult to propagate cuttings due to the low rooting rate.

Method used

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  • Populus diversifolia sexless breeding method based on gene regulatory network
  • Populus diversifolia sexless breeding method based on gene regulatory network
  • Populus diversifolia sexless breeding method based on gene regulatory network

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Embodiment Construction

[0016] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0017] The method for asexual breeding of Populus euphratica based on a gene regulation network according to an embodiment of the present invention will be described below with reference to the accompanying drawings.

[0018] figure 1 It is a flowchart of a method for asexual breeding of Populus euphratica based on a gene regulation network according to an embodiment of the present invention.

[0019] like figure 1 As shown, the Populus euphratica clone breeding method based on the gene regulatory network includes:

[0020] S1...

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Abstract

The invention discloses a populus diversifolia sexless breeding method based on a gene regulatory network. The method comprises the following steps: forming a populus diversifolia positioning group; conducting explant culture according to the populus diversifolia positioning group, and obtaining phenotype data, transcriptomics data, proteomics data and metabonomics data of the populus diversifolia rooting ability according to the populus diversifolia positioning group; positioning QTL, eQTL, pQTL and mQTL of the populus diversifolia rooting ability respectively according to the phenotype data, the transcriptomics data, the proteomics data and the metabonomics data of the populus diversifolia positioning group; forming the gene regulatory network of the populus diversifolia rooting process according to the QTL, the eQTL, the pQTL and the mQTL of the populus diversifolia rooting ability, and positioning network quantitative trait loci (nQTL) of the populus diversifolia rooting ability according to the gene regulatory network; conducting populus diversifolia sexless breeding according to the nQTL of the populus diversifolia rooting ability. According to the method of the embodiment, the populus diversifolia single plant with strong rooting ability can be effectively screened out, the reproductive rate of the populus diversifolia is improved, and the stress resistant sexless breeding of the populus diversifolia is greatly promoted.

Description

technical field [0001] The invention relates to the technical field of breeding, in particular to a method for asexual breeding of Populus euphratica based on a gene regulation network. Background technique [0002] Known as the "hero tree", Populus euphratica is the only tree species that is salt-, cold-, drought-, and wind-sand-resistant distributed in the desert areas of northwest my country. However, due to the serious separation of traits of Populus euphratica seedlings, it is not conducive to screening out excellent individual plants of Populus euphratica. Therefore, in order to overcome the shortcoming of the serious separation of characters of Populus euphratica, currently Populus euphratica is mainly bred through asexual reproduction. However, the rooting ability of Populus euphratica is relatively poor, and the cutting propagation is very difficult due to the low rooting rate when cutting. Therefore, solving the rooting problem of Populus euphratica has become a ...

Claims

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Application Information

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IPC IPC(8): G06F19/18
Inventor 邬荣领张健薄文浩郭允倩徐放
Owner BEIJING FORESTRY UNIVERSITY
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