Application of multi-functional PAP pen in cell climbing immunohistochemical detection

A technology of immunohistochemistry and immunohistochemistry pen, which is applied in the application field of multi-purpose immunohistochemistry pen in immunohistochemistry detection of cell climbing slices, can solve the problem of being difficult to handle large scale, and achieve consistent standards and easy control, Effects of volume reduction, comparability and increased reliability

Active Publication Date: 2015-02-18
绍兴橡树企业管理咨询合伙企业(有限合伙)
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Problems solved by technology

[0003] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical Experimental Scientific Research

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  • Application of multi-functional PAP pen in cell climbing immunohistochemical detection
  • Application of multi-functional PAP pen in cell climbing immunohistochemical detection
  • Application of multi-functional PAP pen in cell climbing immunohistochemical detection

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Embodiment Construction

[0037] An application of a multipurpose immunohistochemical pen in the immunohistochemical detection of cell slides, cells are inoculated in a glass slide culture dish for cell slides, and immunocytochemical staining is carried out after the cell culture is completed; the said The slide-type petri dish is equipped with a petri dish body 1 and a dish cover 2. The petri dish body is divided into two types: single-hole chamber and multi-hole chamber; the area of ​​each hole chamber is larger than the slide glass, and the bottom 3 of the dish body is directly used as a glass slide , the outer surface of the bottom of the dish body is provided with a rectangular frame mark line groove 4 corresponding to the size of the glass slide, and the bottom of the dish body can be pulled out along the rectangular frame mark line groove under the action of an external force;

[0038] The immunocytochemical staining identification includes the following steps in turn:

[0039] (1) Fix with ice ...

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Abstract

The invention discloses an application of a multi-functional PAP pen in cell climbing immunohistochemical detection. The application comprises the following steps: inoculating cells in a glass slide-type culture dish for enabling cells to grow on the glass slide-type culture dish; after cell culture, conducting immunohistochemical dyeing identification. The glass slide-type culture dish is provided with a culture dish body and a culture dish cover, wherein the culture dish body comprises a single-pore chamber model and a porous chamber model; the area of each pore chamber is greater than that of the glass slide; the bottom of the culture dish body can be directly used as the glass slide; a rectangular streak line groove, the size of which is corresponding to that of the glass slide, is formed in the outer side surfaces of the bottom of the culture dish body; the bottom of the culture dish body can be pulled out of the rectangular streak line groove under the action of external force. The application is suitable for various immunohistochemical dyeing experiments on a glass slice, can remarkably reduce antibodies and the reagent dosage, avoids liquid flow and diffusion in dyeing, improves the operation speed, and is particularly suitable for large-scale large-sample and multi-group immunohistochemical dyeing in experimental study of cell growing on the glass slide-type culture dish or cell smearing.

Description

technical field [0001] The invention relates to the application of an immunohistochemical pen in immunohistochemical detection of cell slides. Background technique [0002] Using specific antibodies to conduct in situ qualitative, localization or quantitative research on the distribution of certain chemical components and their content in tissue slices and cell slides, this technique is called immunocytochemistry (immunocytochemistry) technique. [0003] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical experiment scientific research. Contents of the invention [0004] The purpose of the present invention is to provide a multi-purpose immunohistochemical pen suitable for various immunohistochemical staining experiments, which can significan...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/31G01N33/53
CPCG01N1/30G01N1/31G01N33/53
Inventor 鄂群江明田健王海英王东林李春笋陆锦标蒋春峰朱顺星储成存刘小飞焦丽燕刘丽丽曹靖晨张冲颜民邬迪张小川唐妙
Owner 绍兴橡树企业管理咨询合伙企业(有限合伙)
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