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Helicoverpa armigera V-ATPase A gene cDNA and application thereof

A cotton bollworm and gene technology, applied in the field of insect molecular biology and physiology, can solve the problem of few siRNA silencing pest target genes, etc., and achieve the effect of a wide range of hosts

Inactive Publication Date: 2015-02-11
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on silencing pest target genes by siRNA

Method used

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  • Helicoverpa armigera V-ATPase A gene cDNA and application thereof
  • Helicoverpa armigera V-ATPase A gene cDNA and application thereof
  • Helicoverpa armigera V-ATPase A gene cDNA and application thereof

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Embodiment Construction

[0026] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0027] The present invention clones the cDNA of V-type ATPase A subunit (V-ATPase A) from cotton bollworm, which has the sequence shown in SEQ ID NO.1:

[0028] (1) Information of SEQ ID NO.1

[0029] (a) Sequence features:

[0030] Sequence length: 1667 base pairs

[0031] Type: nucleic acid

[0032] Chain type: double chain

[0033] Topology: Linear

[0034] (b) Molecular type: cDNA

[0035] (c) Assumption: No

[0036] (d) Antisense: No

[0037] (e) Original source: Cotton bollworm (Helicoverpa armigera)

[0038] (f) Sequence description: SEQ ID NO.1

[0039] (2) Information of SEQ ID NO.2

[0040] (a) Sequential features

[0041] Sequence length: 555 amino acids

[0042] Type: amino acid

[0043] Chain type: single chain

[0044] Topology: Linear

[0045] (b) ...

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Abstract

The invention discloses helicoverpa armigera V-ATPase A gene cDNA and application thereof. Helicoverpa armigera V-ATPase A siRNA is designed and synthesized, after helicoverpa armigera takes V-ATPase A siRNA in, expression of V-ATPase A gene is blocked, growth and metabolism of helicoverpa armigera are inhibited, the mortality is raised, and therefore helicoverpa armigera V-ATPase A gene cDNA is applicable to biological control of helicoverpa armigera.

Description

technical field [0001] The invention belongs to the field of insect molecular biology and physiology, in particular to the cloning of the cDNA of the V-ATPase A subunit (V-ATPase A) of the cotton bollworm, the design, synthesis and identification of the small RNA (siRNA) of the V-ATPase A gene of the cotton bollworm Feeding of cotton bollworm larvae. Background technique [0002] Cotton bollworm (Helicoverpa armigera), a species of Noctuidae insects, is an important borer pest of cotton buds and bolls. It mainly eats buds, flowers, and bolls, and also feeds on young leaves. Cotton bollworm is a pest with worldwide distribution. It is distributed and damaged in every cotton area in China, and the northern cotton area suffers more seriously than the southern cotton area. Cotton bollworm is a polyphagous pest. In addition to damaging cotton, it can also damage crops such as wheat, corn, sorghum, soybean, pea, alfalfa, sesame, tomato, pepper, and sunflower. The host plants are ...

Claims

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Application Information

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IPC IPC(8): C12N15/55C12N9/14C12N15/10A01N57/16A01P7/04
Inventor 毛建军曾凡荣
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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