Immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies and preparation method of test paper
A technology of anti-nuclear matrix protein and immunochromatographic test paper, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of high cost, complicated operation, low sensitivity, etc., and achieve the effect of fast and intuitive results and easy operation
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Embodiment 1
[0039] combined with figure 1 , 2 : Immunochromatographic test paper for rapid detection of bladder cancer by using anti-nuclear matrix protein 22 (NMP22) antibody, said test paper includes sample pad 1, binding pad 2, nitrocellulose membrane 3, absorbent paper 4 and bottom plate 5; said nitrocellulose The plain film 3 is in the middle of the bottom plate 5, and one end of the nitrocellulose membrane 3 along the bottom plate is sequentially a binding pad 2 and a sample pad 1, and the other end of the nitrocellulose membrane 3 along the bottom plate is an absorbent paper 4 , and every adjacent part overlaps between the two; the nitrocellulose membrane 3 comprises a detection area coated with an anti-nuclear matrix protein 22 antibody and a quality control area coated with an anti-IgG antibody; the nitrocellulose One end of the film 3 near the absorbent paper 4 is a quality control area, and the other end is a detection area; the detection area is provided with a detection line...
Embodiment 2-3
[0041] The following examples 2-3 are the preparation method of the immunochromatographic test paper for rapid detection of bladder cancer by using anti-nuclear matrix protein 22 antibody
Embodiment 2
[0043] 1) Prepare the sample pad, binding pad, nitrocellulose membrane and absorbent paper;
[0044] 2) Preparation of colloidal gold solution: 100ml 0.01% HAuCl 4 Heat the aqueous solution to boiling, quickly add 10ml of sodium citrate solution under vigorous stirring, keep boiling for 30 minutes, then stop heating, and obtain colloidal gold solution after cooling.
[0045] 3) Adjust the pH of the colloidal gold solution obtained in step 1) to 7.0 with an alkaline solution, mix the anti-nuclear matrix protein 22 antibody and the colloidal gold solution to combine them, and then add a protein protective agent to obtain a stable gold-labeled antibody.
[0046] 4) Purify the obtained stable gold-labeled antibody by high-speed refrigerated centrifugation, and redissolve with a certain volume of colloidal gold suspension.
[0047] 5) Spray the gold-labeled antibody onto the binding pad, and after drying, the gold-labeled antibody binding pad is obtained.
[0048] 6) Spray an ant...
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