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Immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies and preparation method of test paper

A technology of anti-nuclear matrix protein and immunochromatographic test paper, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of high cost, complicated operation, low sensitivity, etc., and achieve the effect of fast and intuitive results and easy operation

Inactive Publication Date: 2015-02-04
ZHEJIANG ORIENT GENE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnosis and postoperative review of bladder cancer mainly rely on cystoscopy and urine cytology examination. Although the former is accurate, it is an invasive examination, which is complicated, time-consuming, laborious, expensive, and brings great pain to the patient. Many patients are resistant to this method, and it is difficult to find carcinoma in situ or flat carcinoma; urine cytology is non-invasive and has good specificity, but low sensitivity. It is sensitive to tumors with lower stages and better differentiation. Only 30% or lower, and it is greatly affected by the subjective factors of the diagnostician
The limitations of cystoscopy and urine cytology limit the application of bladder cancer diagnosis, so it is of great significance to find a simple, non-invasive and early diagnosis of bladder cancer experimental method

Method used

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  • Immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies and preparation method of test paper
  • Immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies and preparation method of test paper
  • Immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies and preparation method of test paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] combined with figure 1 , 2 : Immunochromatographic test paper for rapid detection of bladder cancer by using anti-nuclear matrix protein 22 (NMP22) antibody, said test paper includes sample pad 1, binding pad 2, nitrocellulose membrane 3, absorbent paper 4 and bottom plate 5; said nitrocellulose The plain film 3 is in the middle of the bottom plate 5, and one end of the nitrocellulose membrane 3 along the bottom plate is sequentially a binding pad 2 and a sample pad 1, and the other end of the nitrocellulose membrane 3 along the bottom plate is an absorbent paper 4 , and every adjacent part overlaps between the two; the nitrocellulose membrane 3 comprises a detection area coated with an anti-nuclear matrix protein 22 antibody and a quality control area coated with an anti-IgG antibody; the nitrocellulose One end of the film 3 near the absorbent paper 4 is a quality control area, and the other end is a detection area; the detection area is provided with a detection line...

Embodiment 2-3

[0041] The following examples 2-3 are the preparation method of the immunochromatographic test paper for rapid detection of bladder cancer by using anti-nuclear matrix protein 22 antibody

Embodiment 2

[0043] 1) Prepare the sample pad, binding pad, nitrocellulose membrane and absorbent paper;

[0044] 2) Preparation of colloidal gold solution: 100ml 0.01% HAuCl 4 Heat the aqueous solution to boiling, quickly add 10ml of sodium citrate solution under vigorous stirring, keep boiling for 30 minutes, then stop heating, and obtain colloidal gold solution after cooling.

[0045] 3) Adjust the pH of the colloidal gold solution obtained in step 1) to 7.0 with an alkaline solution, mix the anti-nuclear matrix protein 22 antibody and the colloidal gold solution to combine them, and then add a protein protective agent to obtain a stable gold-labeled antibody.

[0046] 4) Purify the obtained stable gold-labeled antibody by high-speed refrigerated centrifugation, and redissolve with a certain volume of colloidal gold suspension.

[0047] 5) Spray the gold-labeled antibody onto the binding pad, and after drying, the gold-labeled antibody binding pad is obtained.

[0048] 6) Spray an ant...

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Abstract

The invention belongs to the technical field of in vitro diagnosis and particularly relates to immunochromatographic test paper for rapidly detecting bladder cancer by utilizing anti-nuclear matrix protein 22 antibodies. The immunochromatographic test paper comprises a sample pad 1, a binding pad 2, a nitrocellulose membrane 3, water-absorbing paper 4 and a bottom board 5, wherein the nitrocellulose membrane 3 is arranged at the middle part of the bottom board 5; one end of the nitrocellulose membrane 3 is provided with the binding pad 2 and the sample pad 1 in sequence along the bottom board, and the other end of the nitrocellulose membrane 3 is provided with the water-absorbing paper 4 along the bottom board; the nitrocellulose membrane 3 comprises a detection area coated with the anti-nuclear matrix protein 22 antibodies and a quality control area coated with anti-IgG antibodies; a detection line (T) is arranged in the detection area and a quality control line (C) is arranged in the quality control line; and the binding pad 2 is coated with the colloidal-gold-labeled anti-nuclear matrix protein 22 antibodies or latex-labeled anti-nuclear matrix protein 22 antibodies or colloidal-selenium-labeled anti-nuclear matrix protein 22 antibodies. The immunochromatographic test paper has the characteristics of simplicity, convenience and no invasion in use and can be applied to clinical auxiliary diagnosis and detection of conditions of bladder cancer.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, in particular to an immunochromatographic test paper for rapidly detecting bladder cancer by using an anti-nuclear matrix protein 22 (NMP22) antibody. [0002] Background technique [0003] Bladder tumors are the most common tumors of the urinary system, and more than 90% are transitional cell carcinomas. Transitional cell carcinoma of the bladder is the most common malignant tumor of the urinary system, mostly occurring between the ages of 50 and 70. More than 80% of them are superficial tumors, and 10%-20% develop into invasive bladder cancer. This disease has a high mortality rate, and early detection and diagnosis play an important role in treatment and prognosis. Data show that the 5-year survival rate of patients diagnosed in the early stage of the disease is greater than 90%, while the 5-year survival rate of patients diagnosed in the late stage is only about 15%-30%. At pres...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574
CPCG01N33/57407
Inventor 张华方效良
Owner ZHEJIANG ORIENT GENE BIOTECH
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