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Phlegm paper for protecting nucleic acids (DNA and RNA) in sputum and nucleic acid extraction method

A technology for sputum paper and sputum, which is applied in the field of biology and achieves the effects of convenient use, no pollution risk and simple operation

Inactive Publication Date: 2015-01-07
宁波有成生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a disposable sputum paper for storing sputum samples, which is used to solve the problems of convenient storage of sputum and protection of nucleic acids in sputum

Method used

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  • Phlegm paper for protecting nucleic acids (DNA and RNA) in sputum and nucleic acid extraction method
  • Phlegm paper for protecting nucleic acids (DNA and RNA) in sputum and nucleic acid extraction method
  • Phlegm paper for protecting nucleic acids (DNA and RNA) in sputum and nucleic acid extraction method

Examples

Experimental program
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Effect test

Embodiment

[0031] 10 sputum samples of patients stored in sputum paper were obtained in the hospital, and the samples were stored at room temperature (25-35° C.) for 1-14 days.

[0032] Cut the sputum paper containing sputum into pieces with surgical scissors, put it in a test tube, add the lysate at a ratio of 1:5, and shake it on a shaker for 30 minutes. Place the nucleic acid adsorption column in a collection tube, transfer all the above lysate to the nucleic acid adsorption column, centrifuge at room temperature (12,000 rpm, 1 min), and discard the filtrate. Add 500 μL of washing solution WB1 to the nucleic acid adsorption column, centrifuge at room temperature (12,000 rpm, 1 min), and discard the filtrate. Add 500 μL of washing solution WB2 to the nucleic acid adsorption column, centrifuge at room temperature (12,000 rpm, 1 min), and discard the filtrate. Place the nucleic acid adsorption column in a new 1.5mL nuclease-free centrifuge tube and let it stand at room temperature or ai...

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Abstract

The invention relates to a method for storing integrity of nucleic acids (DNA and RNA) in a sputum sample in the field of biology. The invention provides phlegm paper, which can be used for conveniently storing nucleic acids in the sputum sample at room temperature; nucleic acid protecting liquid is infiltrated on the phlegm paper, and can be used for inactivating nuclease in the sputum sample; DNA and RNA in the sputum are effectively prevented from being degraded, so as to store and transport the sputum sample. At room temperature (25-35 DEG C), the nucleic acids in the sputum sample can be stably kept for 7-14 days. Meanwhile, the invention also provides a corresponding nucleic acid extraction method.

Description

technical field [0001] The invention relates to a method for preserving the integrity of nucleic acid (DNA and RNA) in a sputum sample in the field of biology, in particular to a sputum paper and a nucleic acid extraction method for conveniently storing nucleic acid in a sputum sample at normal temperature. technical background [0002] The inner walls of the human trachea and bronchi are covered with a layer of mucous membrane, and the submucosa contains more mucous glands and serous glands. Under normal circumstances, goblet cells and glands secrete a small amount of mucus to cover the surface of the mucous membrane, which can protect the mucous membrane and keep the tracheal mucous membrane moist, so as to adhere to the dust particles and bacteria inhaled into the trachea and bronchi, and block them. It penetrates deep into the lung tissue. When the trachea, bronchi, and lungs are stimulated by harmful factors or inflamed by pathogenic bacteria, the mucous membrane of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 唐涛周细武邱英华
Owner 宁波有成生物医药科技有限公司
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