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Polypeptides, nucleic acids encoding the polypeptides, and pharmaceutical compositions

A composition and drug technology, applied in the field of pharmaceutical compositions containing the polypeptide, can solve the problems of difficulty in obtaining large-scale production, the lactoferrin activity needs to be further improved, etc., and achieve high promotion of bone cell differentiation and/or proliferation, Ease of production, short molecular weight effect

Active Publication Date: 2019-01-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, lactoferrin has a molecular weight of about 80kDa and contains about 700 amino acids, which makes it difficult to produce on a large scale due to its large molecular weight
Moreover, the activity of lactoferrin still needs to be further improved

Method used

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  • Polypeptides, nucleic acids encoding the polypeptides, and pharmaceutical compositions
  • Polypeptides, nucleic acids encoding the polypeptides, and pharmaceutical compositions
  • Polypeptides, nucleic acids encoding the polypeptides, and pharmaceutical compositions

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0044] (1) Entrust Beijing Hengyu Vision Biotechnology Co., Ltd. to synthesize the polypeptide shown in SEQ ID No: 1, and the 1-27, 1-28, 1-29 of the amino acid sequence shown in SEQ ID No: 2 bit, 1-30 bit, 1-31 bit, 1-32 bit, 1-33 bit, 1-34 bit, 1-35 bit, 1-36 bit, 1-37 bit, 1-38 bit, 1-39 bit bit, 1-40 bit, 1-41 bit, 1-42 bit, 1-43 bit, 1-44 bit, 1-45 bit, 1-46 bit, 1-47 bit, 1-48 bit, 1-49 bit bit, 1-50 bit, 1-51 bit, 1-52 bit, 1-53 bit, 1-54 bit, 1-55 bit, 1-56 bit, 1-57 bit, 1-58 bit, 1-59 bit bit, 1-60 bit, 1-61 bit, 1-62 bit, 1-63 bit, 1-64 bit, 1-65 bit, 1-66 bit, 1-67 bit, 1-68 bit, 1-69 bit bit, 1-70 bit, 1-71 bit, 1-72 bit, 1-73 bit, 1-74 bit, 1-75 bit, 1-76 bit, 1-77 bit, 1-78 bit, 1-79 bit bit, 1-80 bit, 1-81 bit, 1-82 bit, 1-83 bit, 1-84 bit, 1-85 bit, 1-86 bit, 1-87 bit, 1-88 bit, 1-89 bit bit, 1-90 bit, 1-91 bit, 1-92 bit, 1-93 bit, 1-94 bit, 1-95 bit, 1-96 bit, 1-97 bit, 1-98 bit, 1-99 bit 1-100, a total of 75 polypeptides.

[0045] (2) Obtain the polypep...

Embodiment 11

[0049] This example is used to illustrate the effect of the polypeptide of the present invention on promoting osteocyte differentiation and proliferation in vitro.

[0050] (1) Culture and passage of MC3T3-E1 osteoblasts

[0051] Culture: suspend MC3T3-E1 osteoblasts in 10 mL of α-MEM medium (containing 10% (v / v) fetal bovine serum), inoculate them in culture flasks, and place them at 37°C in 5% CO 2 Cultivate in the incubator, change the medium every 24 hours, and change the medium every 2 days thereafter.

[0052] Subculture: When the monolayer of osteoblasts grows to confluence, use a pipette to gently discard the culture medium in the culture bottle, wash off the remaining culture medium in the culture bottle with about 10 mL of PBS buffer solution; add 2 mL of digestion solution (containing 0.25 % (w / v) trypsin in PBS buffer), shake the flask gently to make the trypsin cover the surface of the cells, place it in the incubator, after 4 minutes, blow the cells off the bott...

Embodiment 12

[0059] This example is used to illustrate the effect of the polypeptide of the present invention on promoting osteocyte differentiation and proliferation in vitro.

[0060] According to the method in Example 1.1, the culture and subculture of MC3T3-E1 osteoblasts, the detection of the proliferation activity of MC3T3-E1 osteoblasts and the detection of ALP activity were carried out. The difference is that the detection of the proliferation activity of osteoblasts and the detection of ALP activity During detection, the polypeptide shown in SEQ ID No: 1 was added to make the final concentration 10 μg / mL. The test results are shown in Table 1.

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Abstract

The invention discloses polypeptide. The polypeptide contains an amino acid sequence shown as SEQ ID NO:1; a sequence of the polypeptide is the amino acid sequence shown as SEQ ID NO:2 or segments of the amino acid sequence shown as SEQ ID NO:2. The polypeptide provided by the invention has high activity for promoting osteocyte differentiation and / or proliferation.

Description

technical field [0001] The present invention relates to a polypeptide, a nucleic acid encoding the polypeptide, the application of the polypeptide and / or nucleic acid in the preparation of drugs for promoting bone cell differentiation and / or proliferation and / or treating osteoporosis, and a Pharmaceutical compositions of polypeptides. Background technique [0002] Osteoporosis is a common and frequently-occurring disease of middle-aged and elderly people, especially postmenopausal women. It ranks first in the prevalence of five major diseases of middle-aged and elderly people. The aggravation of the disease is becoming more and more severe. The research and development of drugs for treating osteoporosis, especially drugs for promoting bone cell differentiation and / or proliferation, has become one of the hot topics in the medical field. [0003] Lactoferrin mainly comes from breast milk. It is non-toxic and harmless. It has a significant role in promoting bone cell differen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00C12N15/10A61K38/16A61K48/00A61P19/10A61P19/00
CPCA61K38/00C07K14/79
Inventor 郭慧媛郑丽敏文鹏程任发政
Owner CHINA AGRI UNIV
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