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A kind of culture medium for inducing green spheroids of Alisma fern

A technology of green spheroids and induction medium, which is applied to plant cells, horticulture, botany equipment and methods, etc., to achieve the effects of short induction time, high induction rate, and improved reproduction efficiency

Active Publication Date: 2016-01-20
YUNNAN JICHUANG HORTICULTURAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the tissue culture propagation technology of the green spheroids of Alisma fern, but the induction of the green spheroids of Alisma fern is the key technology

Method used

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  • A kind of culture medium for inducing green spheroids of Alisma fern
  • A kind of culture medium for inducing green spheroids of Alisma fern

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Acquisition of aseptic explants of Alismatis fern

[0021] Inoculate the aseptic spores of Alismatis fern on 1 / 2MS + 30.0g / L sucrose + 7.0g / L agar, pH 5.80 culture medium, the culture conditions are: 20~25℃, the daily light time is 12 hours, the light intensity It is 2000lx, and after cultivating for 6-7 months, young and tender aseptic seedlings can be obtained, and the young and tender aseptic seedlings are used as Alisma fern aseptic explants.

[0022] The method for obtaining the aseptic spores of Erythronia fern is to place 10-15 mg of spores in a 1.5 ml centrifuge tube, pre-wash them three times with sterile water, centrifuge at 6000 r / min for 2 minutes, obtain the spore precipitate, add a volume fraction of 5% NaClO solution to kill Bacteria for 4 minutes, washed with sterile water for 3-5 times to obtain aseptic spores of Alismatis fern.

[0023] (2) Induction of green spheroids of Alisma fern

[0024] Inoculate the sterile explants of Alisma fern obtained...

Embodiment 2

[0044] Embodiment 2 is the same as Embodiment 1 except that the following steps are different, and will not be repeated here.

[0045] (2) Induction of green spheroids of Alisma fern

[0046] Green spheroid induction medium is: 1 / 6MS+TDZ0.6mg / L+NAA0.2mg / L+hydrolyzed casein 600mg / L+sucrose 30.0g / L+agar 7.0g / L, pH 5.70.

[0047] After 2 weeks of cultivation, the shoot tip and petiole swollen base of the aseptic seedlings will grow into green spheroids, and the average induction rate can reach 95%.

[0048] The average survival rate of the plantlets of Alisma alisma in step (6) is 96.67%.

Embodiment 3

[0050] Embodiment 3 is the same as Embodiment 1 except that the following steps are different, and will not be repeated here.

[0051] (2) Induction of green spheroids of Alisma fern

[0052] Green spheroid induction medium is 1 / 5MS+TDZ1.0mg / L+NAA0.3mg / L+hydrolyzed casein 800mg / L+sucrose 30.0g / L+agar concentration 7.0g / L, pH is 5.90.

[0053] After 2 weeks of cultivation, the shoot tips and petiole bases of sterile seedlings swelled to form green spheroids, and the average induction rate could reach 98.33%.

[0054] In step (6), the average survival rate of the tissue-cultured seedlings of Alisma alizima is 100%.

[0055] One Alisma fern green spheroid induced by the above embodiments can reproduce 1.5 to 2 million high-quality Alisma fern seedlings within one year. Preservation of green spheroids of Alisma fern: use the proliferation medium of green spheroids for preservation, and replace the medium every 3 to 4 weeks. The growth medium of green spheroids is: 1 / 6MS+TDZ0.2mg / L...

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Abstract

The invention discloses an induction medium for green spherical bodies of hemionitis arifolia. The induction medium for the green spherical bodies of the hemionitis arifolia is prepared from 0.4-1.0mg / L 1 / 6MS-1 / 4MS+TDZ, 0.1-0.3mg / L NAA, 400-800mg / L casein hydrolysate, 30.0g / L cane sugar and 7.0g / L of agar; the pH value of the induction medium is 5.70-5.90. The induction medium for the green spherical bodies of the hemionitis arifolia is capable of successfully inducing the green spherical bodies of the hemionitis arifolia, short in induction time and excessively high in induction rate; the green spherical bodies of the hemionitis arifolia can be induced every two weeks; the average induction rate can reach 88.33%-98.33%; 1.5 million to 2 million of high-quality hemionitis arifolia seedlings can be bred in one year by using one green spherical body and can be produced all around the year without the limit of seasons. The induction medium for the green spherical bodies of the hemionitis arifolia provides an important manner for breeding commercial hemionitis arifolia seedlings by virtue of the green spherical bodies.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium for inducing green spheroids of Alisma alisma. technical background [0002] Alisma fern (Hemionitis arifolia) belongs to the genus Alisma fern of the gymnosperm family, trade name: wish fern, wish leaf, heart leaf fern. There are 8 species of Alisma genus, and only 1 species of Alisma is produced in Asia. China is the northern limit of the distribution of this species, mainly distributed in Hainan and southern Yunnan. The leaves of Alisma fern are heart-shaped and unique in shape, and have good market application prospects. [0003] Under natural conditions, ferns mainly rely on spores for reproduction, but the cycle of spore reproduction is long, usually 6-18 months, or even longer. [0004] The fern green spheroid refers to a spheroid composed of numerous green particles induced by fern explants, which is essentially a meristem assem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 余蓉培桂敏莫锡君吴学尉蒋亚莲施自明
Owner YUNNAN JICHUANG HORTICULTURAL TECH
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