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Heterozygous genome processing method

A processing method and genome technology, applied in the fields of genome bioinformatics analysis and genetic engineering, can solve the problems of long cycle, high cost, restricting species genome interpretation, etc., and achieve the effect of shortening cycle and reducing cost.

Active Publication Date: 2014-11-26
BGI TECH SOLUTIONS
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a hybrid genome processing method in view of the defects of the traditional hybrid genome map drawing scheme in the prior art that the cost is high and the cycle is long, which seriously restricts the interpretation of the species genome

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Embodiment Construction

[0060] The objects, features and characteristics of the present invention, as well as the operation method of the relevant elements of the structure and the function and combination of parts will become more apparent when reading the following description and the above claims with reference to the accompanying drawings. It is to be expressly understood that the drawings are for purposes of illustration and description only and are not intended as limitations of the invention.

[0061] In the heterozygous genome processing method of the present invention, without any reference sequence, based on the next-generation high-throughput sequencing technology, the target species is sequenced at an ultra-high depth (generally more than 200 times), and then the new version of SOAPdenovo2 assembly software is used , combined with heterozygous recognition processing, to obtain the whole genome sequence map of the target species. Embodiments of the present invention may include whole genom...

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Abstract

The invention discloses a heterozygous genome processing method. The method comprises the following steps: carrying out at least 200 times ultrahigh deep sequencing on target species by using a whole genome shotgun process to obtain an effective read length sequence Reads; carrying out scaffold sequence Scaffold assembling and construction on the effective Reads to obtain a genome map with a redundant sequence; and carrying out heterozygous recognition processing on the genome map with a redundant sequence to remove redundant Scaffold in a heterozygous region and reserve heterozygous region information in order to obtain a whole genome map. The method realizes the rapid assembling of the whole high-quality heterozygous genome map, and shortens the drafting cycle and cost.

Description

technical field [0001] The invention relates to the technical field of genetic engineering and the field of genome bioinformatics analysis. In particular, the invention relates to heterozygous genome processing to obtain a whole genome sequence map of a species. Background technique [0002] Whole-genome de novo sequencing is also called de novo sequencing. A species can be sequenced without any reference sequence, and the whole genome sequence map of the species can be obtained by splicing and assembling using bioinformatics analysis methods. Obtaining the full genome sequence of a species is an important shortcut to speeding up our understanding of that species. [0003] With the launch of the Human Genome Project, the world has taken an important step in genome sequencing; various models of animal and plant nematodes, Arabidopsis, mice, etc. have been sequenced genomes; due to technical limitations, genome sequencing is still in its infancy. After the completion of the ...

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Application Information

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IPC IPC(8): C12Q1/68G16B30/20G16B20/20
CPCC12Q1/6869G16B30/20G16B20/20C12Q2537/165G16B20/00G16B30/00
Inventor 詹东亮
Owner BGI TECH SOLUTIONS
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