Fluorescent quantitative polymerase chain reaction 384-well pcr plate coordinate sample box

Abstract

The invention relates to the technical field of the biological experimental facilities and discloses a coordinate sample injection box of a 384-hole PCR plate for quantitative real-time polymerase chain reaction. The coordinate sample injection box of the 384-hole PCR plate for the quantitative real-time polymerase chain reaction comprises a sample injection box and a base, wherein the sample injection box is a shape like a cuboid with only four side surfaces; the sample injection box is matched with the 384-hole PCR plate in size; the 384-hole PCR plate can be placed in the sample injection box; a movable transverse mark post and a movable longitudinal mark post are arranged on the sample injection box; 1 to 24 figure marks are formed on the transverse mark post, while A to P letter marks are formed on the longitudinal mark post, and each figure and each letter are capable of corresponding to one micropore in the 384-hole PCR plate; the base is used for placement of the 384-hole PCR plate and the sample injection box and can be separated from the sample injection box. The coordinate sample injection box of the 384-hole PCR plate for the quantitative real-time polymerase chain reaction is capable of realizing accurate positioning of every micropore in the 384-hole PCR plate and immediately tracking the position of the sample injection micropores, and is capable of stopping in one position any time without error.

Description

technical field [0001] The invention relates to the technical field of biological experiment equipment, in particular to a fluorescent quantitative polymerase chain reaction 384-hole PCR plate coordinate sample box. Background technique [0002] In conventional biochemical experiments, a PCR plate is a commonly used experimental consumable, which consists of 96 or 384 sample wells, each of which is conical or cylindrical. Fluorescent quantitative polymerase chain reaction (QRT-PCR) 384-well PCR plate, there are 24 microwells with a diameter of 1.5 mm within a horizontal length of 12.8 cm, and 16 microwells within a vertical length of 8.6 cm, usually complete a 384 Adding samples to the well PCR plate requires 1-2 hours of manual work, and requires complete concentration. If you are inattentive or distracted by speaking, once a microwell is missing, superimposed or misplaced, the subsequent microwells will be coherent. sexual error. [0003] There are fully automatic or sem...

AI Technical Summary

Problems solved by technology

[0004] Existing sampling equipment usually has the following disadvantages: 1) The cost is expensive, depending on the degree of automation, the price ranges from 30,000 to 100,000 euros

2) Inconvenient, this type of equipment is usually used for large-scale multi-sample high-throughput gene detection company operation mode, not suitable for routine gene expression analysis in laboratories or laboratories such as universities, research institutes or hospitals

[0005] The disadvantages of the currently commonly used manual sampling method are: 1) The 384 microwells are arranged in a dense manner, making it difficult to ensure accurate sampling

2) It is very easy to miss addition, superimposition or staggered rows and staggered columns of microporous samples

3) After a mistake, the impact is great

4) Time-consuming and mind-consuming

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