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Strong interference resistant homocysteine detection kit

A homocysteine, detection kit technology, applied in biological testing, measuring devices, material testing products, etc., can solve problems such as unfavorable clinical testing applications, biased results, inaccurate testing results, etc. Interference, Sensitivity and Accuracy High, Consistent Effects

Active Publication Date: 2014-10-22
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this detection method is easily interfered by impurities such as pyruvate in serum, resulting in inaccurate detection results. When compared with the chemiluminescence detection kit, the results are biased, which is not conducive to clinical detection applications.

Method used

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  • Strong interference resistant homocysteine detection kit
  • Strong interference resistant homocysteine detection kit
  • Strong interference resistant homocysteine detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 (blank comparative example)

[0045] Component 1 (R1):

[0046] S-adenosylmethionine (SAM) 0.1mM

[0047] NADH 0.3mM

[0048] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 0.5 mM

[0049]Alpha-ketoglutarate 1mM

[0050] Component 2 (R2):

[0051] Hcy methyltransferase (HMTase) 1KU / L

[0052] Glutamate dehydrogenase (GLDH) 1KU / L

[0053] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 0.5KU / L

[0054] Adenosine deaminase (ADA) 5KU / L

[0055] The homocysteine ​​detection kit (enzyme method) described in this example, when in use, is measured by an automatic biochemical analyzer with dual reagent functions, such as Toshiba 40 automatic analyzer, and its operation is shown in Table 1:

[0056] Table 1 Homocysteine ​​detection reagent detection method

[0057]

[0058] Calculation: homocysteine ​​concentration = (determination / min÷standard / min)×C standard

Embodiment 2

[0060] Component 1 (R1):

[0061] S-adenosylmethionine (SAM) 0.1mM

[0062] NADH 0.3mM

[0063] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 0.5 mM

[0064] Alpha-ketoglutarate 5mM

[0065] Ascorbate oxidase 2.5KU / L

[0066] Component 2 (R2):

[0067] Hcy methyltransferase (HMTase) 5KU / L

[0068] Glutamate dehydrogenase (GLDH) 10KU / L

[0069] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 2.5KU / L

[0070] Adenosine deaminase (ADA) 5KU / L

[0071] Cystathionine β-synthase (CBS) 25 KU / L

[0072] Concrete determination method is with embodiment 1.

Embodiment 3

[0074] Component 1 (R1):

[0075] S-adenosylmethionine (SAM) 0.5mM

[0076] NADH 0.5mM

[0077] Tris(2carboxyethyl)phosphine hydrochloride (TCEP) 2.5 mM

[0078] Alpha-ketoglutarate 5mM

[0079] Ascorbate oxidase 2.5KU / L

[0080] Component 2 (R2):

[0081] Hcy methyltransferase (HMTase) 5KU / L

[0082] Glutamate dehydrogenase (GLDH) 5KU / L

[0083] S-adenosyl homocysteine ​​(SAH) hydrolase (SAHase) 2.5KU / L

[0084] Adenosine deaminase (ADA) 5KU / L

[0085] Cystathionine β-synthase (CBS) 30 KU / L

[0086] Concrete determination method is with embodiment 1.

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Abstract

The invention provides a strong interference resistant homocysteine detection kit. The kit comprises a component 1 and a component 2 according to a volume ratio of 240:65. The addition of ascorbic acid oxidase to the component 1 effectively enhances the interference resistance and reduces the influences of pyruvic acid and interference substances on the detection of the kit in order to enhance the reagent detection accuracy; the detection result of the kit provided in the invention is highly consistent with the detection result of a chemiluminescent detection kit; and the strong interference resistant homocysteine detection kit has higher sensitivity and accuracy than routine homocysteine detection kits, enhances the market competitiveness, and is in favor of the reagent market popularization.

Description

Technical field [0001] The present invention involves a kind of cysteine testing kit, which is the field of clinical in vitro testing agents. Background technique [0002] Capitine (also known as high cysteopatheatic acid HomoCyteine, HCY) is sulfur -containing amino acid, which is generated by oxine -based in the cells.In 1969, McCully first associated to severe same -type cysteinemia (THCY concentration of 100-450 μmol / L) with atherosclerotic disease. Severe same -type cysteinemia was due to participation in same cysteine metabolism.Caused by enzyme defects in transposition or sulfur, commonly includes cuscus enzyme composases and metal methal more tetrahydrogen folic acid reduction enzyme defects.Patients with severe same -type cysteine have premature atherosclerosis, hybolic embolism and intellectual disorders.In recent years, people have begun to pay attention to the connection between the same type of cysteine and cardiovascular disease.Corcladine can make small arterial v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6815G01N2333/90235
Inventor 甘宜梧王进谭柏清李进
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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