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Tenascin c and its use in rheumatoid arthritis

A technology for tenascin and arthritis, applied in the field of tenascin C and its use in rheumatoid arthritis, which can solve the problems of impractical direct assessment

Inactive Publication Date: 2017-03-08
OXFORD UNIV INNOVATION LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Determination of bone erosion is based almost exclusively on imaging findings, ultrasound, and doplar studies, as direct assessment of these lesions by biopsy is far from practical

Method used

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  • Tenascin c and its use in rheumatoid arthritis
  • Tenascin c and its use in rheumatoid arthritis
  • Tenascin c and its use in rheumatoid arthritis

Examples

Experimental program
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Embodiment Construction

[0072] Assessment of Tenascin C Levels in Patients with Rheumatoid Arthritis Compared to Normal / Control Individuals

[0073] Sera were obtained from the blood of patients with rheumatoid arthritis as well as from normal individuals and stored at -80°C until use. On the day of analysis, sera were thawed on ice and typically diluted 1:50 in EIA buffer (1% BSA, 0.05% Tween 20 in PBS) for use in ELISA. An ELISA kit was purchased from IBL (Catalog No. 27751) for the detection of high molecular weight variants of human tenascin C. 100 μL of diluted serum was incubated on ELISA plates pre-coated with an unlabeled capture anti-tenascin C antibody in duplicate at 37 °C for 60 min. The ELISA plate (in 0.001% Tween 20 / PBS) was washed 7 times, and HRP-labeled anti-human tenascin C antibody (100 μL) was added to each well. The wells were then incubated at 4°C for 30 minutes, and then washed 9 times with 0.001% Tween 20 / PBS. 100 μL of chromogen (TMB solution provided) was then added to e...

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Abstract

The present invention provides a method of determining the rheumatoid arthritis status of an individual to be tested, or the progression of rheumatoid arthritis, or an appropriate therapy for an individual suffering from rheumatoid arthritis, the method comprising the steps of: (a ) determining the level of tenascin C in a sample from said individual to be tested; and (b) comparing the level of tenascin C determined in step (a) to one or more reference values. Preferably the rheumatoid arthritis referred to is erosive rheumatoid arthritis. Figure 5 is attached when published.

Description

technical field [0001] The present invention relates to the use of tenascin-C as a biomarker for inflammatory disorders, in particular as a serum biomarker for rheumatoid arthritis. In particular, the present invention relates to the use of tenascin C as a biomarker for erosive rheumatoid arthritis. Background technique [0002] Tenascin C is a proinflammatory extracellular matrix glycoprotein (Midwood, et al. (2009), Nat. Med., 15:774-780), which is induced by activation of the pattern recognition receptor TLR4 Inflammatory cytokine synthesis in primary human macrophages and synovial fibroblasts. Tenascin C is a larger hexameric protein with a molecular weight of 1.5 million Daltons. High levels of tenascin C have been found at sites of inflammation such as in the synovial fluid of rheumatoid arthritis patients and in the tumor stroma. [0003] Rheumatoid arthritis is a chronic disease characterized by persistent inflammation, swelling and pain in multiple joints. Over ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/564
CPCG01N33/564G01N2333/78G01N2800/102A61P19/00A61P19/02A61P29/00G01N33/6893C07K16/241C07K16/2887C07K16/248A61K31/519
Inventor 金·苏姗妮·米德伍德
Owner OXFORD UNIV INNOVATION LTD
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