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Hypertension susceptibility related gene locus and detection method thereof

A technology for hypertension and essential hypertension, applied in the fields of molecular biology and medicine, can solve the problems of decreased expression, less research, abnormal expression regulation, etc.

Inactive Publication Date: 2014-10-15
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

[0007] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

Method used

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  • Hypertension susceptibility related gene locus and detection method thereof

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Embodiment 1

[0036] The 5'-end non-coding region-1248 of the essential hypertension susceptibility gene MFn2 gene was detected by sequencing. The above-mentioned hypertension cases and 30 control samples were selected for sequencing to determine the genotype of the 5'-end non-coding region -1248.

[0037] 1. Experimental method

[0038] The PCR sequencing primers still use the above-mentioned PCR primers, and the amplified products are directly sequenced after being purified. The sequencing instrument was ABI's 3130xl genetic analyzer, and the sequence analysis 5.2 analysis software was used for analysis, and the results could also be viewed with chromas.

[0039] 2. Experimental results

[0040] Screenshot of the sequencing result figure 1 shown.

[0041]

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Abstract

The invention discloses a locus related to essential hypertension susceptibility, and discloses a method for detecting the single nucleotide polymorphism of an essential hypertension related gene mitofusin gene 2 (Mfn2) / hyperplasia suppressor gene (HSG). The method includes detecting the genotype of a 5' non-coding region-1248 locus of an individual mitofusin gene 2 (Mfn2) / hyperplasia suppressor gene (HSG). The invention also discloses a corresponding detection kit.

Description

[0001] technical field [0002] The present invention relates to the fields of molecular biology and medicine. More specifically, it involves the detection of the 5' non-coding region-1248bp of the single nucleotide polymorphism (single nucleotide polymorphism, SNP) site of the human mitochondrial fusion gene 2 (Mfn2 gene). The invention also relates to a kit for detecting the SNP site. [0003] Background technique [0004] Essential hypertension (essential hypertension, EH) is a multifactorial and polygenic disease. It is a common and frequent cardiovascular disease caused by environmental and genetic factors, which has a great impact on human health. With the development of molecular medicine, more than 150 hypertension-related genes have been discovered, but the pathogenesis of EH is still not completely clear, and the early diagnosis and prevention of hypertension have not been fully resolved. EH is the result of the joint action of genetic factors and the environmen...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 王佐广彭晓云刘洁琳魏永祥李梅刘雅文杰许秀芳牛秋丽温绍君
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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