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Fluorescence sensor, as well as preparation method and application of sensor and method for detecting Heparin molecule

A fluorescent sensor and molecular technology, applied in the field of fluorescent sensors, can solve problems such as thrombocytopenia, and achieve the effect of achieving sensitivity

Inactive Publication Date: 2014-10-01
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But if overdose during use can cause side effects, such as bleeding and thrombocytopenia

Method used

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  • Fluorescence sensor, as well as preparation method and application of sensor and method for detecting Heparin molecule
  • Fluorescence sensor, as well as preparation method and application of sensor and method for detecting Heparin molecule
  • Fluorescence sensor, as well as preparation method and application of sensor and method for detecting Heparin molecule

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Embodiment 1

[0044] The preparation and application steps of the fluorescent sensor based on SG fluorescent dye and unlabeled DNA conformation conversion are as follows:

[0045] a. The DNA sequence to be purchased (A 40 ) was dissolved in 0.05M Tris-HCl (pH7.0) buffer solution and stored at -4°C for future use.

[0046] b. Configure the corresponding concentration for the substances used such as coralyne, heparin, and SG.

[0047] c, in the presence of DNA A 40 Add SG to the buffer solution and incubate the mixed solution in a dry oven at 35°C for 10 minutes; then add the prepared Coralyne solution and continue to incubate in a dry oven at 48°C for 10 minutes to obtain A 40 -A blend of SG-Coralyne.

[0048] d. Put the prepared Heparin solution into the solution containing A 40 -The buffer solution of the SG-Coralyne mixture was incubated in a dry box at 45°C for 10 minutes. Using the electrostatic interaction between Coralyne and Heparin, a sensor based on the conversion of the SG flu...

Embodiment 2

[0051] A method for preparing a fluorescent sensor based on SG fluorescent dye and unlabeled DNA configuration conversion, comprising the following steps:

[0052] (1) DNA sequence: A 40 , dissolved in buffer solution, and preserved for later use;

[0053] (2) Configure the corresponding concentrations for the substances used such as coralyne, heparin, and SG

[0054] (3) in the presence of DNA A 40 Add SG to the buffer solution and cultivate to obtain a mixed solution;

[0055] (4) Add the prepared Coralyne solution to obtain A 40 -A blend of SG-Coralyne. Use A 40 The electrostatic interaction between SG and SG, the sensor based on the conversion of SG fluorescent dye and unlabeled DNA configuration was successfully prepared

[0056] In step (1), the DNA sequence was dissolved in 0.05M Tris-HCl (pH7.0) buffer solution, and stored at -4°C for future use. The solution configured in step (2) is used after step (2) is constant to volume. Step (3) contains DNA A 40 Add SG t...

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Abstract

The invention relates to a fluorescence sensor as well as a preparation method and application of the sensor, and a method for detecting Heparin molecules. The fluorescence sensor consists of SG fluorescent dye and non-marked DNA (Deoxyribose Nucleic Acid) configuration transition, and by utilizing the static function of the Heparin molecules with Coralyne, the fluorescence sensor is applied to detection on target Heparin molecules. The preparation method comprises the following steps: dissolving DNA in a Tris-HCl buffer solution, adding SG into the buffer solution containing DNA, and culturing, further adding a prepared Coralyne solution, culturing so as to obtain a mixture of DNA-SG-Coralyne, adding the a prepared Heparin solution into the buffer solution containing the mixture of DNA-SG-Coralyne and culturing, thereby obtaining the sensor based on SG fluorescent dye and the non-marked DNA configuration transition. The preparation method of the fluorescence sensor adopts non-marked DNAs and is easy to operate, low in cost and capable of avoiding any chemical marking or modification. The fluorescence sensor has the characteristics of easiness in operation, high sensitivity and low detection limit.

Description

technical field [0001] The invention belongs to the technical field of fluorescent sensors, in particular to a fluorescent sensor using SG fluorescent dye and unlabeled DNA configuration conversion and its application to the detection of target DNA molecules, in particular to a fluorescent sensor, its preparation method, use and detection of Heparin molecules Methods. Background technique [0002] The 21st century is the century of life sciences. As a biologically active substance, polysaccharides play an important role in many aspects, especially in medical applications. The research of polysaccharide bioluminescent sensor has become a hotspot, such as heparin (Heparin). There are many related studies devoted to the detection of Heparin by fluorescence analysis of unlabeled DNA. Heparin has an anticoagulant effect in vivo and in vitro, can prevent and treat venous thromboembolism, and regulate various normal physiological and pathological processes, such as blood coagulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王广凤江红
Owner ANHUI NORMAL UNIV
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