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Production method of azotification nitragin

A nitrogen-fixing rhizobia and production method technology, applied in the field of agricultural microorganisms, can solve the problems of productization, slow commercialization process, large-scale cultivation and preservation technology of rhizobia, etc., and achieve long storage time, vigorous growth of live bacteria, Continuous collection of large effects

Inactive Publication Date: 2014-09-17
都江堰惠农生物技术有限责任公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the large-scale cultivation and preservation technology of rhizobia has not been overcome, and its productization and commercialization have been progressing very slowly.

Method used

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with embodiment:

[0027] The specific operation process is as follows:

[0028] (1) Prepare the aerobic fermenter. A large-scale plastic pool with a depth of about 1 meter is selected, and air pipes are evenly arranged at the bottom, and the air is supplied by an air pump. The gas passes through an air filtration system to minimize bacteria and impurities in the air. Ensure that the dissolved oxygen in the culture medium is not less than 6mg / l. Fill with 4 / 5 volume of distilled water.

[0029](2) Prepare the non-woven fabric. The material is a soft, porous and dense thin-layer chemical fiber (PVC fiber cotton) with a specification of 30X10X3cm (length X width X thickness), which is evenly arranged in the water body of the fermenter. The basic parameters of each carrier are measured as follows: geometric surface area 840cm2, specific gravity 0.8±0.1kgnf3, specific surface area ≥ 93.3m2m-...

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Abstract

The invention discloses a production method of an azotification nitragin. The production method comprises the following steps of arranging soft fibers in an aerobic fermenter, promoting rhizobium adhesion and growth by a low-molecular organic carbon source-containing medium, regularly supplying a low-molecular organic carbon source and nitrogen source, regularly eluting rhizobia on the soft fibers, storing the collected liquid under the conditions of low-dissolved oxygen, low-pH value and seal environment, carrying out detection, and carrying out packaging by a package vessel. The azotification nitragin obtained by the production method can grow exuberantly and has a large continuous-collection amount, simple preservation conditions and long preservation time. The production method can realize productization and commercialization of rhizobia (liquid). In actual use, the azotification nitragin is directly mixed with seeds and thus use is very convenient.

Description

[0001] technical field [0002] The invention relates to a production method of a nitrogen-fixing rhizobia agent, and the product is mainly used as a microbial agent for planting leguminous crops, and belongs to the technical field of agricultural microorganisms. Background technique [0003] Root nodule bacteria are a type of rod-shaped bacteria that live in symbiosis with legumes, form root nodules and fix nitrogen in the air for plant nutrition. This symbiotic system has a strong ability to fix nitrogen. Rhizobium invades through root hairs, lateral root forks (such as peanuts) or other parts of leguminous plants, forming invasion lines, entering the root cortex, stimulating host cortical cell division, and forming root nodules. Rhizobium enters root nodule cells from the invasion line, Continue to multiply, and the cell group containing rhizobia in the root nodule constitutes the bacteria-containing tissue. After entering these host cells, rhizobia are surrounded by a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C05G3/00
Inventor 李朝均
Owner 都江堰惠农生物技术有限责任公司
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