Use of annelid haemoglobin for maintaining stem cells in the undifferentiated state
A hemoglobin, annelid technology, applied in animal cells, vertebrate cells, non-embryonic pluripotent stem cells, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0034] Materials and Methods
[0035] active agent
[0036] Extracellular hemoglobin from annelids (in this case the green clamworm) was used at test concentrations of 0.25, 0.75 and 1.25 mg / mL in a buffer solution containing 90 mM NaCl, 23 mM sodium gluconate, 2.5 mM CaCl 2 , 27mM sodium acetate, 1.5mM MgCl 2 , 5 mM KCl, and a pH of 7.1±0.5, which may contain 0-100 mM ascorbic acid and / or reduced glutathione type antioxidants. This hemoglobin is commercially available from Hemarina Corporation under the reference number Hemarina-M201.
[0037] CFE Clonogenic Assay (2D)
[0038]Rationale: The CFE Clonogenic Efficacy Assay assesses the frequency of keratinocytes capable of producing cell clones within a given population. A strong clonogenic potential is thought to be associated with stem / progenitor cells, whereas differentiated keratinocytes lose this potential. This culture test consists in seeding keratinocytes at a low density (100 to 1000 cells / dish) in order to o...
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com