Fluid system of flow cytometer and flow cytometric detection method

Abstract

The invention discloses a fluid system of a flow cytometer and a flow cytometric detection method. The fluid system comprises a sampling needle, a sampling needle cleaning device, a flowing chamber, a reaction tank, a hemolytic agent syringe, a sample syringe, a diluent syringe, a diluent bottle, a hemolytic agent bottle and a motor, wherein the sample syringe is respectively communicated with the sampling needle, the reaction tank, the flowing chamber, the sampling needle cleaning device and the diluent syringe, the diluent syringe is respectively communicated with the diluent bottle, the flowing chamber and the reaction tank, the hemolytic agent syringe is respectively communicated with the hemolytic agent bottle and the reaction tank, and the hemolytic agent syringe, the sample syringe and the diluent syringe are all driven and controlled by the motor. According to the invention, driving motors for a plurality of syringes are integrated into one, arrangement of the syringes satisfies moment balance, integral rigidity and inertia are improved, gap instability of the driving motor can be buffered, a more stationary sample flow can be obtained, and thus, detection precision of the flow cytometer is improved.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to a flow cytometer fluid system and a flow cytometry detection method. Background technique [0002] Flow cytometry is a blood analysis instrument that controls cells to pass through the detection area one by one through flow cytometry. Flow cytometry means that the cell flow (also called sample flow) is wrapped in the sheath fluid, and the cells line up through the flow chamber detection area. Advanced fluid control technology, which can avoid the problem of inaccurate detection caused by cells passing through the detection area according to irregular routes, and can greatly reduce the probability of missed detection caused by overlapping cells passing through the detection area. [0003] Existing flow cytometry is mainly divided into two categories. The first category is that both the sheath flow and the sample flow are pushed by a syringe. The second type is that the sample f...

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Problems solved by technology

[0009] In view of the above deficiencies in the prior art, the purpose of the present invention is to provide a flow cytometer fluid system and a flow cytometry detection method, aiming to solve the problems of the existing flow cytometer system complexity, high cost, etc.

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