Water culturing and transplanting method for apple tissue culture seedlings
A tissue culture seedling and hydroponic technology, applied in the field of plant tissue culture, can solve problems such as poor transplanting effect, and achieve the effects of low cost, simple implementation, and improved transplanting survival.
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Embodiment 1
[0017] Apple tissue culture technology includes the establishment of explants, multiplication culture, rooting culture, transplanting and other links, of which:
[0018] Establishment of explants: Cut the top buds or side buds of annual young apple trees that grow vigorously and finely in the field, rinse with tap water, disinfect the surface with 74% alcohol, sterilize with 0.1% mercury liter, and finally rinse with sterile water Finally, the induction medium is connected to the induction medium on the ultra-clean workbench of the sterile room, and the induction differentiation is carried out in the culture room, and the non-toxic shoot tip is obtained as an explant for culture;
[0019] Expansion and cultivation: Cut the non-toxic shoot tips obtained by the above method into 2cm stem segments, inoculate them into the expansion medium on the ultra-clean workbench in the sterile room, pick 4 buds from each bottle of medium, and put them in the cultivation room. Proliferation; ...
Embodiment 2
[0030] Apple tissue culture technology includes the establishment of explants, multiplication culture, rooting culture, transplanting and other links, of which:
[0031] Establishment of explants: Cut the top buds or side buds of annual young apple trees that grow vigorously and finely in the field, rinse with tap water, disinfect the surface with 74% alcohol, sterilize with 0.1% mercury liter, and finally rinse with sterile water Finally, the induction medium is connected to the induction medium on the ultra-clean workbench of the sterile room, and the induction differentiation is carried out in the culture room, and the non-toxic shoot tip is obtained as an explant for culture;
[0032] Expansion and cultivation: Cut the non-toxic shoot tips obtained by the above method into 2cm stem segments, inoculate them into the expansion medium on the ultra-clean workbench in the sterile room, pick 3 buds from each bottle of medium, and place them in the cultivation room Proliferation;...
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