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Application of allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells in prevention or treatment of rheumatoid arthritis

A rheumatoid, vascular layer technique

Pending Publication Date: 2014-08-06
CELLULAR BIOMEDICINE GRP SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some RA patients cannot provide autologous fat due to various reasons, such as those with infectious diseases or being old and thin, which makes it impossible to use autologous adipose-derived mesenchymal progenitor cells to prevent or treat RA

Method used

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  • Application of allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells in prevention or treatment of rheumatoid arthritis
  • Application of allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells in prevention or treatment of rheumatoid arthritis
  • Application of allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells in prevention or treatment of rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0156] SVF and haMPCs in the treatment of rheumatoid arthritis (RA)

[0157] The patient is a 53-year-old female patient who has experienced repeated swelling and pain in the interphalangeal joints and metacarpophalangeal joints for more than 5 years. The morning stiffness lasted for 1 hour. The laboratory test of RF352RU / ml and anti-CCP565RU / ml was diagnosed as rheumatoid arthritis.

[0158] After oral treatment with non-steroidal anti-inflammatory drugs and leflunomide, the symptoms were not relieved. SVF and haMPCs were performed after the consent of the patients. Because the patient is thin, BMI is 16.8kg / m 2 Therefore, allogeneic SVF and allogeneic haMPCs are recommended for treatment.

[0159] The volunteers for liposuction were 32-year-old women who underwent liposuction to lose weight in plastic surgery, and excluded infectious diseases and chronic diseases such as heart, liver, lung, and kidney. Fat was extracted from the lower abdomen, and about 35ml of fat was ta...

Embodiment 2

[0162] Identification of embodiment 2SVF and haMPCs

[0163] 1. Flow detection

[0164] Cells were collected into centrifuge tubes by enzymatic digestion, and the cell suspension was adjusted to a density of 1×10 5 / mL, 800r / min (120g), centrifuge for 5min, discard the supernatant, wash the resuspended cells with cold D-Hanks at 4°C, centrifuge the cell suspension again at 800r / min for 5min, and then discard the supernatant. Then the cells were resuspended to 1 mL with D-Hanks, 5-10 μL of antibody was added, protected from light, and placed on ice for 30 min. Rinse with D-Hanks, centrifuge, discard the supernatant, and repeat the washing process 2-3 times to ensure that unbound antibodies are removed. Finally, add about 200 to 300 μL of D-Hanks to make a suspension.

[0165] The flow cytometric detection results of SVF are shown in Table 1.

[0166] Table 1

[0167]

[0168] The expression of cell surface antigen markers on SVF was analyzed by flow cytometry. The result...

Embodiment 3

[0179] haMPCs stimulation test

[0180] (1) Fresh and frozen haMPCs were cultured in complete medium and 5% FBS medium respectively, and the VEGF secreted by haMPCs was detected.

[0181] The results show( figure 2 A), in the fresh haMPCs group cultured in complete medium, the concentration of VEGF decreased with the increase of LPS concentration; in the fresh haMPCs group cultured in 5% FBS medium at 200ng / ml, the concentration of VEGF was basically the same as that of the control group, 100ng / ml and 300ng / ml decreased respectively, and the concentration of VEGF in the cryopreserved haMPCs group cultured in complete medium basically changed little with the increase of LPS concentration. On the whole, the VEGF of serum culture is higher than that of complete medium.

[0182] (2) Detected the secretion of VEGF of haMPCs under hypoxia stimulation, and found that the secretion of VEGF under hypoxia stimulation was 2-3 times that of normal culture, and the secretion of 48 hours...

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Abstract

The invention relates to application of allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells in prevention or treatment of rheumatoid arthritis. Specifically speaking, the pharmaceutical composition containing allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells derived from allogenic fat is applied to an object in need, exerts a substantial prevention or treatment effect on rheumatoid arthritis and has good security and no rejection reaction. The invention further provides the pharmaceutical composition containing allogenic stromal vascular fraction cells and allogenic mesenchymal progenitor cells and a method for preventing and treating rheumatoid arthritis.

Description

technical field [0001] The invention belongs to the field of stem cells and biomedicine. Specifically, the present invention relates to the application of allogeneic interstitial vascular layer cells and allogeneic mesenchymal progenitor cells in the prevention or treatment of rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (Rheumatoid Arthritis, RA) is a common chronic inflammation in the world caused by autoimmune disorders, the immune system attacks the joints, and its pathological features are chronic inflammatory hyperplasia of the joint synovium, pannus formation , cartilage and subchondral bone destruction, eventually leading to joint deformity and ankylosis. Its prevalence accounts for about 1% of the world's total adult population, and it is about 0.32% to 0.36% in my country. The average life expectancy of patients is shortened by 5 to 10 years, and at least 50% of patients lose their working ability 10 years after the onset of disease. ...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61K39/00A61K38/18A61K38/19A61K38/20A61P29/00A61P19/02
Inventor 曹卫曾晓聆M·A·卡尤姆周玉洁张丽
Owner CELLULAR BIOMEDICINE GRP SHANGHAI
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