Nanotechnology for myocardial infarction diagnosis and device adopting same

Abstract

The invention discloses a nanotechnology for myocardial infarction diagnosis and a device adopting the nanotechnology. The detection device comprises a sample feeding region, a reagent storage region, a micro-channel and a detection region arranged on the micro-channel, wherein the sample feeding region is provided with a filter membrane for filtering blood cells; a nano magnetic bead-marked anti-human cTnI (cardiac troponin I) monoclonal antibody and a nano fluorescent probe-marked anti-human cTnI monoclonal antibody are stored in the reagent storage region; the reagent storage region is communicated with the detection region through the micro-channel; a magnetic device is arranged below the detection region; a fluorescent detection device is arranged above the detection region. The detection device is used for quantitatively detecting cTnI, and is short in detection time, small in size and convenient to carry.

Description

[0001] technical field [0002] The invention relates to a nanotechnology and its device for myocardial infarction diagnosis. Background technique [0003] There are many kinds of myocardial infarction marker proteins, and cardiac troponin I (cTnI) is known as the gold marker protein of myocardial infarction. Troponin is composed of three subunits of troponin I, T, and C, and plays an important regulatory role in the process of muscle contraction and relaxation. When the myocardium is injured, the cardiac troponin complex is released into the blood. Among the three subunits, troponin I has a high degree of myocardial specificity and sensitivity, so cardiac troponin I (cTnI) has become the most ideal markers of myocardial infarction. [0004] Routine detection methods for cardiac troponin I include radioimmunoassays (RIAs), high-pressure liquid chromatography, electrochemical analysis, protein analyzers, various diagnostic kits, and immunochromatographic test strips, etc. T...

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Problems solved by technology

[0004] Routine detection methods for cardiac troponin I include radioimmunoassays (RIAs), high-pressure liquid chromatography, electrochemical analysis, protein analyzers, various diagnostic kits, and immunochromatographic test strips, etc. These methods have radioactive contamination , the need for large instruments, long time-consuming, quantitative detection and other shortcomings, the clinical application has certain limitations, and it is not easy to popularize and use

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