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The method of using sponge homogenate to stimulate macroalgae to increase the extracellular release of photosynthesis products

A technology for photosynthesis and homogenate, which is applied in the fields of botanical equipment and methods, seaweed cultivation, horticultural methods, etc., can solve the problems of high extraction cost of waste, affecting the health of marine ecosystems, etc., to avoid high extraction costs and market prospects. expansive effect

Inactive Publication Date: 2016-01-27
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction of the above photosynthetic products from macroalgae requires the harvesting of macroalgae, which affects the health of marine ecosystems. On the other hand, the extraction cost is high and a large amount of waste is generated.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] For the experiment that the luxuriant membrane sponge homogenate stimulated the macroalga Ulva pore to increase the extracellular release of photosynthetic products, seven 5-L Erlenmeyer flasks were filled with 2.5-L sterile sand-filtered seawater (110°C, 10min), of which 6 Put into 5.1g, 5.0g, 5.2g, 5.0g, 5.2g and 5.2g Ulva pore respectively in 6 conical flasks, and add luxuriant membrane sponge homogenate to 3 of these 6 conical flasks respectively to reach the content 10mg-sponge homogenate / L-sterilized seawater, shake the Erlenmeyer flask to mix the sponge homogenate evenly, which is used as the experimental group; the remaining 3 of the 6 conical flasks are used as the control group. Finally, in the remaining 1 conical flask containing only sterilized sand-filtered seawater, add luxuriant membrane sponge homogenate to a content of 10 mg-sponge homogenate / L-sterilized sand-filtered seawater, shake the conical flask to make the sponge homogenate Mix well and serve as...

Embodiment 2

[0024] For the experiment in which the luxuriant membrane sponge homogenate stimulates the macroalgae carrageen to increase the extracellular release of photosynthetic products, the experimental procedure is the same as that in Example 1, except that carrageen is used instead of Ulva pore. The final experimental results showed that 10mg / L and 50mg / L of the homogenate of the luxuriant membrane sponge respectively stimulated about 5.0g carrageen (in 2.5-L sterilized sand-filtered seawater) to release organic carbon into the extracellular space within 8 hours The mean increases were 17.5mg and 21.6mg, respectively.

Embodiment 3

[0026] For the experiment in which the kidney finger sponge homogenate stimulates the large seaweed Ulva pore to increase the extracellular release of photosynthetic products, the experimental procedure is the same as that of Example 1, except that the kidney finger sponge is used instead of the luxuriant membrane sponge to prepare the sponge homogenate. The experimental results showed that 10 mg / L and 50 mg / L of kidney finger sponge homogenate respectively stimulated about 5.0 g of Ulva pore (in 2.5-L sterilized sand-filtered seawater) to release extracellular organic carbon within 8 hours on average. An increase of 15.7mg and 20.1mg, respectively.

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PUM

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Abstract

The invention discloses a method for utilizing sponge homogenate to stimulate macroalgae to add extracellular release photosynthesis products. The method is characterized by comprising the steps of breaking sponge, utilizing sterilized sand filter seawater to make the sponge homogenate, and enabling the concentration to be 200mg of sponge in fresh weight per mL of sterilized seawater; placing the macroalgae in a container containing the enough sterilized sand filter seawater, also placing the sponge homogenate in the container, evenly mixing the mixture until the concentration is at least 10mg of sponge homogenate per L of sterilized sand filter seawater, and performing cultivation at 21 DEG C to 24 DEG C. The method stimulates the macroalgae to add the extracellular release photosynthesis products while keeping the macroalgae in a survival and growth state, and marine biological resources can be utilized sustainably and environmentally friendly.

Description

technical field [0001] The invention relates to a method for using sponge homogenate to stimulate macroalgae to increase extracellular release of photosynthetic products. Background technique [0002] Macroalgae convert inorganic salts and water into their own biomass through photosynthesis. Usually people extract carbohydrates, protein and other resource products and natural active products from macroalgae. However, the extraction of the above-mentioned photosynthetic products from macroalgae requires the harvesting of macroalgae, which affects the health of the marine ecosystem. On the other hand, the extraction cost is high and a large amount of waste is generated. Therefore, it is expected to achieve the purpose of sustainable utilization of marine biological resources by stimulating macroalgae to increase extracellular release of photosynthetic products while maintaining macroalgae in a living and growing state. Contents of the invention [0003] The present inventi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00A01G7/06
CPCY02A40/80
Inventor 付晚涛苏延明王刚
Owner DALIAN OCEAN UNIV
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