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Method for producing ethanol by virtue of simultaneous saccharification and fermentation of starch-based biomass

A technology of simultaneous saccharification and fermentation, biomass, applied in the direction of biochemical equipment and methods, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., to improve saccharification and fermentation efficiency, reduce dosage, reduce cost effect

Inactive Publication Date: 2014-07-02
JILIN DESIGNING INST OF CNPC NORTHEAST REFINING & CHEM ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the problem that the saccharification process of starch-based biomass is inhibited in the existing step-by-step saccharification and fermentation technology, the present invention proposes a method for synchronous saccharification and fermentation of starch-based biomass to produce ethanol, comprising the following steps:

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  • Method for producing ethanol by virtue of simultaneous saccharification and fermentation of starch-based biomass

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Embodiment 1

[0026] A method for synchronous saccharification and fermentation of starch-based biomass to produce ethanol, said method specifically comprising the steps of:

[0027] Take cornstarch with a crushed particle size of 0.3-0.6mm, after high-temperature sterilization, add 15U / g α-amylase and 150U / g glucoamylase, and then mix it with sterile water at a ratio of 1g:3g, and then carry out Feed to the saccharification tank for saccharification at a temperature of 80°C and a pH of 4.5. At the same time, part of the saccharification liquid is transported to the filter device by the centrifugal pump. The pore size of the separation membrane is 0.2 μm, and the filtration method is cross-flow. After filtration, the solid phase (mainly incompletely saccharified starch) is returned to the saccharification tank. The clarified liquid rich in monosaccharides is sent to a fermentation tank containing a high temperature resistant yeast culture at a fermentation temperature of 30°C and a pH of 5...

Embodiment 2

[0029] A process for synchronous saccharification and fermentation of starch-based biomass to produce ethanol, the method specifically comprising the steps of:

[0030] Take the wheat starch that has been mechanically crushed and sterilized by high temperature, add 20U / g α-amylase and 120U / g glucoamylase, then mix it with sterile water at a ratio of 1g:2.8g, and then feed it into the saccharification kettle. Saccharification was carried out under the conditions of temperature 80°C and pH=4.5. At the same time, part of the saccharification liquid is transported to the filter device by the centrifugal pump. The pore size of the separation membrane is 0.2 μm, and the filtration method is cross-flow. After filtration, the solid phase (mainly incompletely saccharified starch) is returned to the saccharification tank. The clarified liquid rich in monosaccharides is sent to a fermentation tank containing a high temperature resistant yeast culture at a fermentation temperature of 30°...

Embodiment 3

[0032] A process for synchronous saccharification and fermentation of starch-based biomass to produce ethanol, the method specifically comprising the steps of:

[0033] Take cassava starch that has been mechanically crushed and sterilized at high temperature, add 12U / g of α-amylase and 120U / g of glucoamylase, then mix it with sterile water at a ratio of 1g:2.5g, and then feed it into the saccharification kettle. Saccharification was carried out under the conditions of temperature 80°C and pH=4.5. At the same time, part of the saccharification liquid is transported to the filter device by the centrifugal pump. The pore size of the separation membrane is 0.2 μm, and the filtration method is cross-flow. After filtration, the solid phase (mainly incompletely saccharified starch) is returned to the saccharification tank. The clarified liquid rich in monosaccharides is sent to a fermentation tank containing a high temperature resistant yeast culture at a fermentation temperature of...

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Abstract

The invention provides a method for producing ethanol by virtue of simultaneous saccharification and fermentation of a starch-based biomass. The method comprises the steps of saccharifying the starch-based biomass by using an alpha-amylase and a saccharifying enzyme in a saccharification kettle; collecting accharified liquor after enzymolysis; and fermenting the saccharified liquor by using a yeast culture medium in a fermentation kettle to obtain a fermentation liquor containing ethanol. The method is characterized in that one part of the saccharified liquor is collected from the saccharification kettle and conveyed to a filtering device during the saccharification process, a solid phase obtained after filtering returns to the saccharification kettle, a liquid phase obtained after filtering is conveyed to the fermentation kettle, and meanwhile, the fermentation liquor of the same volume circularly returns to the saccharification kettle. The invention also provides a device for producing ethanol by virtue of simultaneous saccharification and fermentation of the starch-based biomass. The method and the device have the advantages that the inhibition effect on the alpha-amylase and the saccharifying enzyme due to the accumulation of glucose in a hydrolysate in a step-by-step saccharification and fermentation process is avoided, the dosage of the enzymes is reduced and the cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of biomass energy and resources, and in particular relates to a method for synchronous saccharification and fermentation of starch-based biomass raw materials to produce ethanol. Background technique [0002] With the rapid development of industrialization, there will inevitably be energy shortages. Therefore, there is an increasing interest worldwide in developing new alternative energy sources. Bioethanol is the main form of alternative energy and the most viable substitute for fossil energy. The biomass raw materials used to produce ethanol by fermentation are mainly divided into three categories according to traditional methods: sugar, starch and cellulosic substances. At present, the technology of producing fuel ethanol from starch-based biomass (such as corn, wheat, cassava, etc.) is the most mature and has the highest market share. [0003] The main process routes for preparing fuel ethanol from st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12M1/00
CPCY02E50/17Y02E50/10
Inventor 祝涛张慧艾衍林周江沛丁旭杨昌南张军郭华苑文博郭晓宇张洪刚潘磊
Owner JILIN DESIGNING INST OF CNPC NORTHEAST REFINING & CHEM ENG
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