System and method for constructing injected three-dimensional cell microenvironment based on microfrozen gel

Abstract

The invention discloses a system and a method for constructing an injected three-dimensional cell microenvironment based on microfrozen gel. A microfrozen gel three-dimensional microenvironment vector is made of the frozen gel and in micro scale size. Experiments demonstrate that the prepared microfrozen gel three-dimensional microenvironment vector has the following characteristics that mechanical properties are good; whole morphology can be kept after being injected; the vector can load cells effectively; compared with conventional large-scale frozen gel, the microfrozen gel three-dimensional microenvironment vector can absorb the cells uniformly and has good material transmission performance; the microfrozen gel three-dimensional microenvironment vector has a protection effect for loaded cells in an injection process; and the microfrozen gel loaded with the cells can be injected into an animal body at specific sites and keep original functions and activities in in-vivo cells. The microfrozen gel three-dimensional microenvironment vector plays an important role in the field of cell injection therapy, and has wide application prospects in animal model establishment and the drug screening field.

Description

technical field [0001] The invention provides a system and method for constructing an injection-type three-dimensional cell microenvironment based on microcryogel. Background technique [0002] Regenerative medicine represented by cell therapy has gradually become an important direction in the development of clinical medicine, bringing new hope for complex and serious diseases that are difficult to be effective in drug treatment. Cell therapy is a treatment method that uses the patient's own (or allogeneic) adult cells or stem cells to repair tissues and organs. At present, there have been a large number of reports on the use of cells to treat chronic diseases such as cardiovascular diseases, diabetes, Parkinson's disease, corneal lesions, cartilage tissue damage, and tumors, and preliminary results have been achieved in clinical applications. In particular, many breakthroughs have been made in the research and application of stem cell therapy. Stem cells are a type of plu...

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Problems solved by technology

There are four reasons why direct injection causes a large number of transplanted stem cells to die and lose after entering the body: (1) During the injection and transplantation of stem cells, due to the influence of mechanical shear force in the syringe, some cells rupture, and the cells enter the apoptotic stage, reducing cell viability

(2) The free stem cells in vitro enter the damaged tissue area in the body. During this process, the microenvironment of the cells changes, and the microenvironment of the lesion area is not conducive to cell survival and growth, and the stem cells cannot adapt to the microenvironment of the lesion. thereby reducing the viability of the cells

(3) Stem cells are directly injected into the body. Due to the difference between the treatment site and the injection site, some injection sites may be due to mechanical movement or pressure difference between the inside and outside of the tissue site (such as prevertebral disc herniation disease model, cartilage tissue injury model, cardiopulmonary repair transplantation, etc.) and other models), the cells injected into the target tissue will overflow from the needle hole as the syringe is pulled out, resulting in a large number of cell loss. Oriented dissociation, unable to actively aggregate, resulting in the loss of a large number of transplanted cells, unable to perform fixed-point cell therapy

Therefore, this simplified two-dimensional microenvironment cannot simulate and reproduce the three-dimensional microenvironment in the body well.

However, depending on the stem cells cultured in the traditional two-dimensional environment, during the stem cell transplantation treatment, due to the drastic changes in the living environment in vivo and in vitro, the survival rate and function of stem cells are reduced, and the repair and regeneration functions are reduced, which restricts the use of stem cell therapy. The effect of clinical application

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