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Bacillus amyloliquefaciens and bismerthiazol compound wettable bactericidal powder and application thereof

A technology of amylolytic spores and wettable powders, which can be used in applications, biocides, disinfectants, etc. It can solve problems affecting rice quality, excessive pesticide residues, environmental pollution, etc., to reduce pesticide costs, improve control effects, and improve farmland The effect of ecological environment

Active Publication Date: 2014-06-25
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to: use chemical pesticides in large quantities to prevent and control bacterial diseases in rice production, especially the increasing area of ​​rice bacterial streak and bacterial blight. Excessive chemical agent control is likely to cause environmental pollution, resulting in excessive pesticide residues , affecting the quality of rice, a wettable fungicidal powder compounded with Bacillus amyloliquefaciens thiacumazole, which has a good control effect on rice bacterial diseases, and its application in the control of rice bacterial diseases

Method used

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  • Bacillus amyloliquefaciens and bismerthiazol compound wettable bactericidal powder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation of 10% Thiacumazole · 3 billion Bacillus amyloliquefaciens wettable powder

[0018] Effective drugs: 99% thiacumazole original drug (Zhejiang Longwan Chemical Co., Ltd.) and 6 billion Bacillus amyloliquefaciens wettable powder (Jiangsu Suke Agrochemical Co., Ltd.).

[0019] Auxiliary selection: Tween 20.

[0020] Filling accessories: light calcium carbonate

[0021] Each component is prepared according to weight percentage:

[0022] 99% Thiacumazole original drug: 10.1%; 6 billion Bacillus subtilis wettable powder: 50%; Tween 20: 1%; the rest is made up to 100% with light calcium carbonate.

[0023] Preparation process: Put Thiacumazole and Bacillus amyloliquefaciens in the mixer according to the above ratio, add Tween 20, fill it with light calcium carbonate to 100%, mix all the materials in the mixer evenly, and pass through the jet mill Pulverized, it becomes 10% Thiacumazole · 6 billion Bacillus amyloliquefaciens wettable powder.

Embodiment 2

[0025] Determination of Synergistic Effect of 10% Thiacumazole·3 Billion Bacillus amyloliquefaciens WP on Inhibiting the Growth of Rice Stripe Bacteria

[0026] Tested strains: Xoc b5-16 of Xoc b5-16 was provided by Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.

[0027] Medication to be tested: 10% Thiacumazole·3 billion Bacillus amyloliquefaciens wettable powder provided by Example 1.

[0028] Contrast drugs:

[0029] A.20% thiacumazole wettable powder (provided by Zhejiang Longwan Chemical Co., Ltd.);

[0030] B. 6 billion Bacillus amyloliquefaciens wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).

[0031] Indoor synergistic bioassay:

[0032] The Xoc b5-16 strain of rice stripe blight to be tested was activated on NA slant medium (each L contains 3.0g of beef extract, 5.0g of peptone, 2.5g of glucose, and 18.0g of agar), and a loop was picked and inoculated into NA liquid Incubate at 28°C for 24 hours in the culture medium, an...

Embodiment 3

[0044] Determination of synergistic effect of 10% thiacumazole·3 billion Bacillus amyloliquefaciens wettable powder on the growth of bacterial blight of rice

[0045] The tested strains: Xoo Z173 was provided by the Plant Protection Institute of Jiangsu Academy of Agricultural Sciences.

[0046] Medication to be tested: 10% Thiacumazole·3 billion Bacillus amyloliquefaciens wettable powder provided by Example 1.

[0047] Contrast drugs:

[0048] A.20% thiacumazole wettable powder (provided by Zhejiang Longwan Chemical Co., Ltd.);

[0049] B. 6 billion Bacillus amyloliquefaciens wettable powder (provided by Jiangsu Suke Agrochemical Co., Ltd.).

[0050] Indoor synergistic bioassay:

[0051] The Xoo Z173 strain of rice stripe blight to be tested was activated on the NA slant medium, and a ring was picked and inoculated into the NA liquid medium and cultivated at 28°C for 24 hours, and the bacterial solution was diluted to 10 8 cfu / ml, draw 200μl and spread it evenly on the NA...

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Abstract

The invention relates to bacillus amyloliquefaciens and bismerthiazol compound wettable bactericidal powder and application thereof. A finished agent comprises effective active ingredients, aids and filling auxiliary materials, and is characterized in that the effective active ingredients account for 10-85 percent by weight of the finished agent and consist of bacillus amyloliquefaciens and bismerthiazol; in the effective active ingredients, the weight percent of bacillus amyloliquefaciens is 10-90 percent. During application, the finished agent is diluted and uniformly sprayed to rice leaves for preventing and treating rice Bacterial streak or bacterial blight of rice. The finished agent has the advantages that bacillus amyloliquefaciens and the chemical agent, bismerthiazol, are mixed according to a certain ratio, a synergistic effect of microbes and chemical agents can be fully exerted, the advantages are complemented with one another, the prevention and treatment range is widened, the dosage of the chemical agent is reduced, the administration cost is reduced, the prevention and treatment effect is improved, the ecological environment is improved, and the residue amount of the chemical agent in rice is reduced.

Description

technical field [0001] The invention relates to a wettable bactericidal powder compounded by Bacillus amyloliquefaciens and thiacumazole and its application, especially a wettable bactericidal powder composed of Bacillus amyloliquefaciens and thiacumazole and its use in preventing and treating bacterial diseases of rice Applications. Background technique [0002] With the rapid development of the national economy and the improvement of people's living standards, higher and higher requirements are put forward for the safety of food products. Rice diseases caused by bacteria are one of the important diseases in rice production in my country, especially in the south, and are also one of the important limiting factors to ensure the safety of rice production in my country. At present, the disease occurs in an area of ​​more than 20 million mu all year round, causing a loss of 5-10% of rice yield, and even more than 30% in severe cases. In traditional rice production, the preven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/14A01P1/00A01N43/824
Inventor 张荣胜陈志谊刘永锋戴秀华王晓宇罗楚平
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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