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A method for improving the recovery rate of indicator bacteria in biological indicator bacteria tablets

A technology of biological indicator and indicator bacteria, applied in the field of microbiology, can solve the problems of poor elution effect and recovery rate of less than 50% to 100%, and achieve the effect of stable results, good repeatability, and perfect disinfection technical specifications

Active Publication Date: 2016-03-02
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the vibration elution method recommended by this specification, the elution effect on the indicator bacteria on the biological indicator bacteria sheet is poor in actual work, and the recovery rate is far below 50% to 100%.

Method used

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  • A method for improving the recovery rate of indicator bacteria in biological indicator bacteria tablets
  • A method for improving the recovery rate of indicator bacteria in biological indicator bacteria tablets
  • A method for improving the recovery rate of indicator bacteria in biological indicator bacteria tablets

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the bacterium sheet crushing method of the present invention improves the recovery rate of the indicator bacteria of the biological indicator bacterium sheet

[0039] 1. Experimental materials and methods

[0040] The biological indicator bacteria sheet for testing: the special biological indicator for chlorine dioxide gas sterilization produced by SGMBiotech Inc. of the United States (the catalog number is SGMG / 6), the indicator bacteria is the black variety of Bacillus subtilis (ATCC9372), and the bacterial load is 1.6×10 6 cfu / tablet. Biological indicators are within the validity period.

[0041] 1. Bacteria sheet crushing method of the present invention (experimental group)

[0042] (1) Crushing treatment

[0043] Take 3 pieces (that is, 3 parallel samples) of the biological indicator bacteria for testing, and place each piece in a 2ml PE round-bottomed centrifuge tube (diameter of the tube opening is 12mm, and the tube length is 40mm) containing 1...

Embodiment 2

[0065] Embodiment 2, the fragmentation method of the bacterial flakes of the present invention improves the recovery rate of the indicator bacteria of the biological indicator flakes

[0066] 1. Experimental materials and methods

[0067] The test biological indicator bacteria tablet: the Sihuan brand biological indicator produced by Beijing Sihuan Sanitary Pharmaceutical Equipment Factory Co., Ltd. The indicator bacteria is the strain preserved by the Disinfection and Testing Center of the Academy of Military Medical Sciences, and it is also the black variety of Bacillus subtilis (ATCC9372). The amount of bacteria is 5×10 5 ~5×10 6 cfu / tablet. Biological indicators are within the validity period.

[0068] (1) Crushing treatment

[0069] Refer to step 1 (1) of Example 1.

[0070] (2) Dilution, filtration and detection

[0071] Refer to step 1 (2) of Example 1.

[0072] 2. Experimental results

[0073] The results showed that the bacterial load of the domestic Sihuan bi...

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Abstract

The invention discloses a method for improving indicator microbe recovery rate of a bionidicator sheet. The method provided by the invention comprises the following steps: 1) placing the bionidicator sheet in a centrifuge tube filled with an eluant, and adding a stainless steel ball, and crushing by a tissue crusher to obtain a crushed sample; 2) carrying out vortex oscillation on the crushed sample, and diluting by the eluant after oscillation to obtain the diluted sample; and 3) filtering the diluted sample by a 100-400-mesh cell sieve and removing sheet chips to obtain the filtrate which is a bacterium suspension of the indicator microbe obtained from the bionidicator sheet. Experiment verifies that the indicator microbe recovery rate of the method reaches up to 83.2%, and the error rates among panels and dilution are less than 10%. The method is stable in result and good in repeatability, and fully meets the related requirements of disinfecting technical specifications. The method is simple and convenient, reliable and feasible. The invention provides a reliable and practical solution for correctly evaluating and judging the disinfecting and sterilizing effect.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to a method for improving the recovery rate of indicator bacteria of biological indicator bacteria tablets. Background technique [0002] At present, it is generally accepted at home and abroad that the verification method for the effectiveness of disinfection and sterilization should be able to prove that the probability and possibility of residual contaminating microorganisms should be less than 10%. -6 [UnitedStatesEnvironmentalProtectionAgency.AssessmentofLiquidandPhysicalDecontaminationMethodsforEnvironmentalSurfacesContaminatedwithBacterialSporesDevelopmentandEvaluationoftheDecontaminationProceduralSteps[S].EPA,2012.] It is an internationally recognized method to use biological indicators to verify the disinfection and sterilization effects. [0003] A biological indicator (bio-indicator, BI) is a product that dyes an appropriate carrier with a certain amount of specific microorgan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
Inventor 朱以萍张瑞丰谢清黄力勤陆兵吕京
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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