Preparation of common antigen monoclonal antibody of Brucella sLPS (lipopolysaccharides) and establishment of c-ELISA (competitive enzyme-linked immuno sorbent assay)method
A monoclonal antibody, Brucella technology, applied in the field of immunology, can solve the problem that the chemical structure is not clearly identified, and achieve the effect of high application value
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[0028] Hereinafter, the present invention will be described in detail with reference to Examples, which are only intended to illustrate the present invention and are not intended to limit the scope of the present invention. The scope of the invention is specifically defined by the appended claims.
[0029] 1 Materials and methods
[0030] 1.1 Main reagents
[0031] Proteinase K, DNase I, RNase, benzidine diamine (OPD), DMEM medium, and PEG3000 were all purchased from Sigma; HAT and HT selection medium were purchased from Gibco; the monoclonal antibody subclass identification kit was purchased from Southern Biotech Company; Protein G Sepharose column was purchased from GE Healthcare Company.
[0032] 1.2 Strains
[0033] Vaccine strains S-19, M5-90 and S2 Brucella, Escherichia coli (O: 157), Salmonella Dublin (C79-86), Yersinia enterocolitica (O: 9) and Scruff Rutella (M111) were all commercially purchased from China Veterinary Drug Control Institute.
[0034] 1.3 Experime...
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