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Homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and kit used for method

A quantitative analysis and homogeneous luminescence technology, which is applied in the field of clinical examination and diagnosis, can solve the problems of complex, unsuitable and high detection cost of biochip technology, and achieve the effect of saving specimen consumption, simple operation and high precision.

Inactive Publication Date: 2014-06-04
TIANJIN NANKAI HOSPITAL
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Problems solved by technology

In addition, biochips are not suitable for the detection of routine clinical specimens due to their complex technology and high detection costs.

Method used

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  • Homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and kit used for method
  • Homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and kit used for method
  • Homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and kit used for method

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Embodiment Construction

[0026] Taking the simultaneous quantitative analysis of serum alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), sugar chain antigen 125 (CA-125), and ferritin (Ferr) as examples, the homogeneous luminescence immunity for simultaneous determination of multiple components is explained in detail. Analysis methods (such as image 3 ): There are four types of acceptor microspheres in the detection system, all of which contain the same chemiluminescence agent and different fluorescein. Among them, 1# acceptor microspheres contain FITC and 2# acceptor microspheres contain PE, 3# receptor microspheres contain EDC, 4# receptor microspheres contain PE-Cy5), each receptor microsphere is coated with a polyclonal antibody, 1# receptor microsphere is anti-AFP, 2 #Receptor microspheres are anti-CEA, 3#receptor microspheres are anti-CA-125, and 4#receptor microspheres are anti-Ferr. In addition, there are four biotinylated monoclonal antibodies in the system, namely Bio-anti-AFP, Bio-a...

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Abstract

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

Description

Technical field [0001] The invention relates to a homogeneous luminescence immunoassay method for simultaneous determination of multiple components, which is characterized in that it can simultaneously quantitatively analyze multiple biomarkers in a sample to be tested, and can be applied to the field of clinical testing and diagnosis. Background technique [0002] Labeled immunoassay is an ultra-trace biological analysis method. It uses the high affinity between the antigen and the antibody and the high measurability of the marker as a probe, which can accurately quantitatively analyze the ultra-trace material in biological samples. The advantages of simple operation, high specificity, and high sensitivity have become one of the important detection methods in the fields of biology and clinical medicine. [0003] Luminol oxygen channel immunoassay (LOCI) technology is a new type of labeled immunoassay. Similar to LOCI technology, Shanghai Boyang Biotechnology Co., Ltd. has also dev...

Claims

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Application Information

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IPC IPC(8): G01N33/533G01N21/64
CPCG01N21/6402G01N33/53G01N33/533
Inventor 常艳敏张盈莹朱黎娜侯丽英李会强吴嫱
Owner TIANJIN NANKAI HOSPITAL
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