A liquid bromelain preparation with stable enzyme activity

A bromelain and enzyme activity technology, applied in the direction of enzyme stabilization, hydrolytic enzymes, etc., can solve the problems of difficult increase in production capacity, high investment in equipment, time-consuming, etc., and achieve the effect of expanding production capacity, less investment in equipment, and convenient use

Inactive Publication Date: 2015-11-18
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, freeze-drying requires a freeze-drying machine, which requires high equipment investment, energy and time consumption, and the production capacity is not easy to increase. In addition, the loss of enzyme activity during the freeze-drying process still reaches 10% to 30%.

Method used

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  • A liquid bromelain preparation with stable enzyme activity
  • A liquid bromelain preparation with stable enzyme activity
  • A liquid bromelain preparation with stable enzyme activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Add 600 grams of sorbitol, 50 grams of glucose, 100 grams of sucrose, and 50 grams of sucrose to the 200 grams of pineapple enzyme aqueous solution (the content of bromelain is 4%) that has been preliminarily purified, concentrated and filtered sterilized. The sorbitol, glucose, sucrose and sucrose are food grade or pharmaceutical grade. Stir at room temperature (25°C) (100 r / min) for 60 minutes. After all the added enzyme activity protecting agent is dissolved, adjust the pH to 3 with citric acid solution (2mol / L), and measure the enzyme activity within 2 hours. The enzyme activity was measured after being stored at 0°C for 6 months, and the enzyme activity decreased by 1.8%; the enzyme activity was measured after being stored at 0°C for 6 months, and the enzyme activity decreased by 3.9%.

Embodiment 2

[0019] 520 grams of sorbitol, 150 grams of glucose, 50 grams of sucrose and 50 grams of sucrose fructose were added to 230 grams of pineapple enzyme aqueous solution (5% bromelain content) that had been preliminarily purified, concentrated and filtered. The sorbitol, glucose, sucrose and sucrose are food grade or pharmaceutical grade. Stir at room temperature (25°C) (90 r / min) for 60 minutes. After all the added enzyme activity protecting agent is dissolved, adjust the pH to 3.5 with citric acid solution (2mol / L), and measure the enzyme activity within 2 hours. The enzyme activity was measured after being stored at 35°C for 6 months, and the enzyme activity decreased by 2.3%; the enzyme activity was measured after being stored at 35°C for 6 months, and the enzyme activity decreased by 4.8%.

Embodiment 3

[0021] Add 500 grams of sorbitol, 150 grams of glucose, 50 grams of sucrose, and 50 grams of sucrose to the 250 grams of pineapple enzyme aqueous solution (2% bromelain content) that has been preliminarily purified, concentrated and filtered. The sorbitol, glucose, sucrose and sucrose are food grade or pharmaceutical grade. Stir at room temperature (25°C) (90 r / min) for 60 minutes. After all the added enzyme activity protecting agent is dissolved, adjust the pH to 5 with citric acid solution (2mol / L), and measure the enzyme activity within 2 hours. The enzyme activity was measured after being stored at 4°C for 6 months, and the enzyme activity decreased by 1.3%; the enzyme activity was measured after being stored at 4°C for 6 months, and the enzyme activity was decreased by 3.5%.

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Abstract

The invention relates to a liquid bromelain, and particularly relates to a liquid bromelain preparation with stable enzyme activity. The liquid bromelain preparation with stable enzyme activity disclosed by the invention is composed of a bromelain aqueous solution, sugar alcohol, monosaccharide, disaccharide and fructose-oligosaccharide. The enzyme activity of the liquid bromelain preparation prepared by the above formula is still higher than 95% after being stored for 12 months at a normal temperature. The liquid bromelain preparation has the characteristics of highly stable enzyme activity, low equipment investment, energy conservation, time saving and low transportation requirements, is convenient for use by users, and can expand capacity.

Description

technical field [0001] The invention relates to a liquid bromelain, in particular to a liquid bromelain preparation with stable enzyme activity. Background technique [0002] Bromelain is a protease that exists in pineapple plants (including fruit, stem, stalk and leaf), and its active center contains sulfhydryl (-SH), which is widely used in medicine, food, cosmetics, textile, feed, leather and other industries. [0003] The extraction and purification methods of protein mainly include salting out, isoelectric point precipitation, organic solvent precipitation, tannin (tannic acid) precipitation, ion exchange chromatography, affinity chromatography, ultrafiltration concentration and other methods. The production process of bromelain mainly includes ammonium sulfate precipitation method, tannin precipitation method, ultrafiltration method, etc. The last step of these methods is a drying process, and the drying methods mainly include freeze drying and vacuum heating drying. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96C12N9/50
CPCC12N9/63C12N9/96
Inventor 谢庆武刘金星何佳丽
Owner GUANGXI UNIV
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