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Method for extracting polyphenol from tobacco through double-water-phase system

A two-phase aqueous system and extraction technology, applied in the field of bioengineering, can solve the problems of low extraction efficiency, large solvent consumption, troublesome separation, etc., and achieve the effect of fast separation, high concentration and less amount of organic solvent

Inactive Publication Date: 2014-06-04
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for extracting polyphenols in tobacco with a two-phase system, which can solve the problems of low extraction efficiency, large solvent consumption, high cost, and high cost in the prior art of extracting polyphenols from tobacco. Subsequent separation troubles and other issues

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Dry and crush the tobacco leaves (fragments), and pass through a 60-80 mesh sieve.

[0015] (2) Weigh 30g of anhydrous ammonium sulfate and dissolve it in 120g of distilled water, then add 50g of absolute ethanol, stir and let stand to form a new two-phase aqueous system.

[0016] (3) Put 20g of powdered tobacco raw material into a circulating ultrasonic extractor, then add 200g of aqueous two-phase extraction solvent prepared in step (2), and extract at 15°C for 45min at 200W ultrasonic power. After the extraction is completed, the feed liquid is released, and it is allowed to stand at room temperature for 240 minutes. The upper phase is an organic phase containing tobacco polyphenols, and the solid slag is mainly distributed in the middle layer and the lower phase. The upper phase was directly absorbed by the siphon method, concentrated in vacuum, washed with absolute ethanol to remove inorganic salts, and dried at low temperature to obtain 0.42 g of tobacco polyp...

Embodiment 2

[0019] (1) Dry and crush the tobacco leaves (fragments), and pass through a 60-80 mesh sieve.

[0020] (2) Weigh 120g of anhydrous dipotassium hydrogen phosphate and dissolve it into 330g of distilled water, then add 150g of anhydrous methanol, stir and let stand to form a new two-phase aqueous system.

[0021] (3) Put 20g of powdered tobacco raw material into a circulating ultrasonic extractor, then add 600g of aqueous two-phase extraction solvent prepared in step (2), and extract at 50°C for 30min at 300W ultrasonic power. After the extraction is completed, the feed liquid is released, and it is allowed to stand at room temperature for 300 minutes. The upper phase is an organic phase containing tobacco polyphenols, and the solid slag is mainly distributed in the middle layer and the lower phase. The upper phase was directly absorbed by the siphon method, concentrated in vacuum, washed with absolute ethanol to remove inorganic salts and dried at low temperature to obtain 0.68...

Embodiment 3

[0023] (1) Dry and crush the tobacco leaves (fragments), and pass through a 60-80 mesh sieve.

[0024] (2) Weigh 72g of anhydrous ammonium sulfate and dissolve it in 208g of distilled water, then add 120g of absolute ethanol, stir and let stand to form a new two-phase aqueous system.

[0025] (3) Put 20g of powdered tobacco raw material into a circulating ultrasonic extractor, then add 400g of aqueous two-phase extraction solvent prepared in step (2), and extract at 40°C for 60min at 100W ultrasonic power. After the extraction is completed, the feed liquid is released, and it is left to stand at room temperature for 200 minutes. The upper phase is an organic phase containing tobacco polyphenols, and the solid residue is mainly distributed in the middle layer and the lower phase. The upper phase was directly absorbed by the siphon method, concentrated in vacuum, washed with absolute ethanol to remove inorganic salts and dried at low temperature to obtain 0.52 g of tobacco polyp...

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Abstract

The invention discloses a method for extracting polyphenol from tobacco through a double-water-phase system. The method comprises the following steps: (1) ultrasonically extracting dry and crushed tobacco raw material, adding an inorganic salt solution and a hydrophilic micromolecule organic solvent to form the double-water-phase system, mixing, stirring, ultrasonically extracting, standing to separate the phases, wherein the polyphenol is extracted to the upper phase and the residues are distributed to the lower phase and a demarcation layer; and (2) directly sucking the organic solvent upper phase in the double water phase after ultrasonic extraction in the step (1), so as to obtain polyphenol extract; removing the inorganic salt from the polyphenol extract, and drying at a low temperature to obtain a tobacco polyphenol product. On premise of same material-liquid ratio, the one-time extraction efficiency is higher than that of a pure ethanol solution, and moreover a small amount of organic solvent is used, the organic solvent is rapid to separate, a solid residual material does not need to be filtered, the contents of protein and sugar in the extracted organic phase are greatly reduced, the obtained extraction liquid is high in concentration, small in volume, beneficial to later concentration and purification, and beneficial to industrialization.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically refers to a method for extracting polyphenols in tobacco with a two-phase system. The invention relates to a method for extracting polyphenol compounds from tobacco, in particular to a method for extracting polyphenols in tobacco with a two-phase system. Background technique [0002] In recent years, polyphenolic compounds have become a hot spot in the research of natural plant resources. The functions of tea polyphenols and grape polyphenols have been widely accepted by people. The elimination of free oxygen and other body regulation functions are closely related. Tobacco also contains a large amount of polyphenols, the highest content of which is chlorogenic acid (greater than 3%). At present, people's understanding of polyphenols in tobacco is mostly related to smoking. It is found that polyphenols are important aroma precursors in tobacco, but there are still relativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/81
Inventor 张旗孙德平毛耀黄龙姚元军
Owner HUBEI CHINA TOBACCO IND
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