Use of beggarweed alkaloid monomer component

A technology of alkaloids and leech, applied in the field of alkaloid monomers

Inactive Publication Date: 2014-05-28
LANZHOU UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no total alkaloids and its monomer components bufonaline, 5-Hydroxy-N,N-dimethyltryptamine N12-oxide, 5-methoxy-N, Use of N-dimethyltryptamine in the preparation of monoamine oxidase inhibitors

Method used

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  • Use of beggarweed alkaloid monomer component
  • Use of beggarweed alkaloid monomer component
  • Use of beggarweed alkaloid monomer component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 The alkaloid monomer components of leeches alkaloids bufynine, 5-Hydroxy-N, N-dimethyl tryptamine N 12 Preparation of -oxide, 5-methoxy-N,N-dimethyltryptamine

[0025]10kg of leeches, ground into powder, reflux extraction with 6 times of industrial ethanol in 80°C water bath twice, each time for 2 hours. After the extraction is completed, the ethanol extracts are combined, filtered, and the filtrate is evaporated to an extract sample under reduced pressure. The alcohol extraction extract is suspended in 10L of distilled water, and after stirring, the suspension is adjusted to about pH=2-3 with 2mol / L hydrochloric acid solution. After the acid solution was left to stand for 8 hours, filter it, adjust the filtrate to pH=10-11 with 4mol / L sodium hydroxide, immediately extract it three times with an equal volume of chloroform, combine the organic phases, and evaporate to dryness under reduced pressure to obtain the alkaloid extract . A total of 7.5g. Perform m...

Embodiment 2

[0026] Example 2 Determination of the monoamine oxidase inhibitory activity of the alkaloid monomer components of the leeches by enzyme labeling method

[0027] Determination of the alkaloid monomer components of the mountain leeches by enzyme labeling method: compounds 1 - 3 (Concentrations are all 100 μ g / ml) inhibition rate, iproniazid phosphate is the positive control drug. The specific experimental process is as follows:

[0028] (1) Preparation of monoamine oxidase:

[0029] One female wistar rat was killed by cervical dislocation, the liver was taken out, cut into pieces, washed several times with phosphate buffer (pH=7.6), after washing, 20ml of 0.3M sucrose buffer was added for homogenization After fully homogenizing, take 10ml each and pour into two 50ml centrifuge tubes. After washing the homogenizer with 20ml of 0.3M sucrose buffer, 10ml of each was poured into the aforementioned centrifuge tubes. After balancing with a tray balance, centrifuge at 1000×g for 1...

Embodiment 3

[0036] Example 3 Forced swimming test (depressed mouse model)

[0037] Put male SD rats individually into a glass cylinder with a water depth of 15cm (height: 40cm and diameter: 18cm), the water temperature is (24±2)°C, and the water depth can be slightly adjusted so that the rear feet of the rats can just touch the bottom of the cylinder but are not enough to support Body is appropriate. When first put in, the rats were forced to swim and struggled to climb up or dive into the bottom of the cylinder. After 2-3 minutes, the activity gradually weakened, and the intermittent immobility state appeared, and the time became longer and longer. About 80% of the total time, the rats were dried after 15 minutes of the pre-test. Experimental grouping: excluding the unqualified ones in the preliminary test, 50 qualified rats were randomly divided into 5 groups, 10 rats in each group. The blank control group and the fluoxetine positive control group were intragastrically given normal sal...

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Abstract

The invention discloses use of a beggarweed alkaloid monomer component. A beggarweed alkaloid monomer contains bufotenin, 5-Hydroxy-N,N-dimethyltryptamineN12-oxide and 5-methoxy-N,N-dimenthyl tryptamine. The use of the beggarweed alkaloid monomer component is used for preparing a monoamine oxidase inhibitor, namely the use in preparation of a medicament for preventing and treating depressive disorder and Parkinsonism.

Description

technical field [0001] The present invention relates to the use of alkaloid monomers. Background technique [0002] With the rapid development of the global economy and the aggravation of population aging, modern people's life pressure is increasing, and there are more and more patients with depression and Parkinson's disease. Therefore, the study of drugs for the treatment of depression and Parkinson's disease (monoamine oxidase inhibitor drugs) has aroused widespread interest. For finding such drugs, medicinal plants are a very rich resource, and their medicinal ingredients have the advantages of good curative effect, small side effects, and wide range of actions. [0003] Mountain leech ( Desmodium racemosum ) is a plant of the genus Fabaceae, which grows on the roadside at the foot of the mountain, under the sparse forest on the low hillside, on the edge of the forest, on the edge of the field or in the grass. Distributed in Jiangsu, Anhui, Zhejiang, Jiangxi, Fujian, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4045A61P25/24A61P25/16
Inventor 杨中铎智康康
Owner LANZHOU UNIVERSITY OF TECHNOLOGY
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