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A breeding method for increasing the proportion of female bees of the red-necked beetle

A technology of the red-necked brachypter and proportion, which is applied in animal husbandry and other fields, can solve problems such as inconsistency, and achieve the effect of expanding the number of female bees

Active Publication Date: 2016-05-04
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the different ecological status of each organism and the characteristics of the species, the ability to adapt to the environment is also very different, so it cannot be said in the same way.

Method used

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  • A breeding method for increasing the proportion of female bees of the red-necked beetle
  • A breeding method for increasing the proportion of female bees of the red-necked beetle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The host is an old Lygus nymph with a body length of about 2.8-3.2 mm and a width of about 1.4-1.6 mm. Put 50-100 such green Lygus nymphs into 1.2 liters of cylindrical insect culture boxes, and put into 3-4 pairs of red-necked wasps that have emerged for 2-5 days at the same time. The time of inoculation was 1 day. Put 2 fresh green beans, 8-12 fresh corns, and 2 moist cotton balls containing 10% honey water in the insect raising box. Put the cylindrical insect culture box on the glass insect culture rack in the inoculation room, let the light source upwards, and illuminate the bottom of the insect culture box through the glass. Cover the upper part of the insect culture box with a black cloth and seal it with a rubber band. Adjust the temperature in the inoculation room to 22-24°C, and turn on the lights for 14 hours a day.

[0014] After the inoculation was completed, the parasitoid wasps in the insect-raising box were removed, and the parasitized Lygus chinensis w...

Embodiment 2

[0016] (1) Select the appropriate host age for vaccination

[0017] Select 80 healthy, strong nymphs of Lygus nymphs with a body length of about 3.2-3.4 mm, and 3 pairs (3 males and 3 females) of red-necked wasps that have been eclosion for 3 days and put them into a round insect rearing box. (diameter 11cm, height 12cm). At the same time, 10 corn kernels, 2 green beans and cotton balls containing 10% honey water were put into the insect-raising box for feeding on Lygus nymphs and parasitic wasps. When inoculating, the insect rearing box was placed at 23°C, and the parasitoid wasps were allowed to inoculate for 1 day. The control light time is 14 hours.

[0018] Table 1 Effects of ages of Lygus nymphs on the sex ratio of parasitoids

[0019]

[0020] The preferred body length of the present application is 2.8-3.4 mm.

[0021] (2) Select the appropriate temperature for parasitoid breeding

[0022] The inoculated Lygus nymphs were transferred to the pupation box and cont...

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Abstract

The invention relates to the field of artificially feeding natural enemy insects, in particular to a breeding method for improving the female bee proportion of red-neck normal temperature room braconidae bees. The method includes the steps that (1), a suitable host instar is selected for inoculation, 50-100 green plant bug nymphs which are 2.8 mm-3.4 mm long and 1.4 mm-1.6 mm wide are placed in an insect breeding box, meanwhile 3-4 pairs of red-neck normal temperature room braconidae bees 2 days-5 days after eclosion are placed in the insect breeding box, the time for inoculation is one day, the temperature is adjusted at 22 DEG C-24 DEG C, and the lamp is turned on for fourteen hours per day; (2), parasitic wasps are bred, after inoculation, the parasitic wasps in the insect breeding box are rejected, and the green plant bug nymphs on which parasitism is performed continue to be bred with temperature controlled at 25 DEG C-27 DEG C until maturing red-neck normal temperature room braconidae bee larvas get out of the body wall of hosts and fall into vermiculite for cocooning and pupating. The female proportion in parasitic wasp population can be improved, so that the quality of the artificially fed parasitic wasp population is improved, and the parasitic wasps are applied more effectively to field release to safely and effectively control injurious insects.

Description

technical field [0001] The invention relates to the field of artificial breeding of natural enemy insects, in particular to a breeding method for increasing the proportion of female bees of the red-necked beetle. Background technique [0002] Mirid bugs are an important class of worldwide pests, causing serious damage to pastures and various fruit trees. Apolygus lucorum is the most important species among the species of Lygus that harm crops in my country. [0003] The nymph parasitoid of the mirid bug is recognized as an effective natural enemy. The red-necked chrysalis is a unique natural enemy resource in my country, and it has a high parasitism rate to the nymphs of the family Miridae such as the green Lygus. There are four stages in the development of Braconus red-necked wasp, larva, pupa and adult. The female wasp lays eggs into the host body, and the eggs and larvae of the parasitoid develop in the host body and feed on the nutrients in the host body. When the pa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 罗淑萍吴孔明张峰陆宴辉李红梅
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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