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Unfreezing liquid for frozen equine animal embryo, and preparation method and application thereof

A technology of freezing and thawing and thawing liquid, which is applied in the field of freezing and thawing liquid of equine animal embryos and its preparation, can solve the problems of cumbersome preparation steps, high price, and difficult sources of medicines, and achieve simple preparation, convenient material extraction, and facilitate popularization and application Effect

Inactive Publication Date: 2014-04-30
QINGDAO DERUI JUNFA BIOLOGY TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of the traditional embryo freezing and thawing solution, such as difficult source of drugs, high price, cumbersome preparation steps, and the need for immediate use and preparation, the present invention provides a non-surgical embryo freezing method suitable for large-scale production of equine animal embryo transfer. Thawing liquid and its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 27g of sodium lactate, 16g of sodium chloride, 23g of potassium chloride, 5.5g of calcium chloride and 1.6g of magnesium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 6 hours, and set aside; weigh 0.4g of astragalus polysaccharide Put it into a sterile beaker, add it to the spare mixed solution into the sterile beaker, stir to fully dissolve the astragalus polysaccharide, and obtain a sodium lactate mixed solution; before cryopreservation of the embryos, add penicillin, Streptomycin and calf serum can be used to obtain embryo freezing and thawing solution.

[0023] Use the embryo freezing and thawing solution of this embodiment to freeze and thaw non-surgical bovine embryos.

[0024] Example 2

Embodiment 2

[0026] Weigh 10.5g of sodium lactate, 6g of sodium chloride, 13.5g of potassium chloride and 9g of calcium chloride, add 5000ml of distilled water to mix, place in a thermostat at 37°C for 3 hours, and set aside; weigh 1.4g of astragalus polysaccharide into Add it to the sterile beaker, add it to the spare mixed solution, stir to fully dissolve the astragalus polysaccharide, and get the sodium lactate mixed solution; before the embryo is frozen and thawed, add penicillin and streptomycin to the sodium lactate mixed solution according to the component content And inactivated horse serum, the embryo freezing and thawing solution can be obtained.

[0027] The embryo freezing and thawing solution in this example is applied to the freezing and thawing of non-surgical Fama embryos.

[0028] Example 3

Embodiment 3

[0030] Weigh 26g of sodium lactate, 10.8g of sodium chloride, 21g of potassium chloride and 16.8g of calcium chloride, add 4000ml of distilled water to mix, and place it in a thermostat at 37°C for 1 hour to obtain a sodium lactate mixture for use; before the embryo is thawed According to the component content, penicillin, streptomycin and inactivated horse serum are added to the sodium lactate mixture to obtain the embryo freezing and thawing solution.

[0031] The embryo freezing and thawing solution in this example is applied to the freezing and thawing of non-surgical Fama embryos.

[0032] Example 4

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PUM

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Abstract

The invention discloses an unfreezing liquid for frozen equine animal embryos, and a preparation method and application thereof. The unfreezing liquid includes a sodium lactate mixed solution, penicillin, streptomycin, and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum. The preparation method includes the steps of: placing the sodium lactate mixed solution in a thermostat at 37 DEG C for 0.5-6 h for standby; and adding penicillin, streptomycin and one, two or three selected from bovine serum albumin, calf serum and inactivated equine serum into the sodium lactate mixed solution in accordance with the component contents, so as to obtain the unfreezing liquid for frozen embryos. The unfreezing liquid is suitable for non-surgical method for embryo transplantation of horses, cattle and donkeys. The invention has the beneficial effects of convenience for material acquisition, low price, simple preparation and convenience for usage, is very suitable for scale production of non-surgical method for embryo transplantation of equine animals, and is favorable for popularization and application of embryo transplantation technology.

Description

technical field [0001] The invention belongs to the field of animal embryo transplantation, in particular to an equine animal embryo freezing and thawing liquid and its preparation method and application. Background technique [0002] Embryo freezing technology is to put embryos and freezing liquid into cryogenic tubes, and through slow (2-3 day embryos) and fast (5-6 day blastocyst) cooling methods, the embryos can be stilled and can be frozen. A method of preservation in liquid nitrogen at -196 degrees. In the process of embryo transfer, if there are remaining embryos with good quality, they can be frozen and stored, and then implanted into the uterine cavity after natural cycle or artificial cycle after thawing, which will increase the chance of conception. [0003] Embryo freezing and thawing liquid is a protective nutritional liquid for embryos during the process of thawing embryos. At present, there are few reports on the preservation of embryo freezing and thawing s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 邹敬清张志鹏李成修
Owner QINGDAO DERUI JUNFA BIOLOGY TECH
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