Fertility recovery method for oyster distant hybrid sterility

A technology for distant hybrids and fertility restoration, which can be applied in fish farming, application, climate change adaptation, etc., and can solve the problems of oyster distant hybrid sterility and breeding work cannot be carried out

Active Publication Date: 2014-04-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

[0005] The object of the present invention is to provide a method for restoring fertility of oyster distant hybrid sterility, which solves the problem that subsequent breeding work cannot be carried out due to oyster distant hybrid sterility. The self-breeding potential evaluation of hybrid progenies provides data and references, and also provides a possible way for the development of new varieties

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  • Fertility recovery method for oyster distant hybrid sterility
  • Fertility recovery method for oyster distant hybrid sterility

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Embodiment 1

[0017] The first step, the preparation of hybrid F1: using the interspecific hybridization model, using Hong Kong oyster and long oyster of the genus Macrophylla as materials, firstly the Hong Kong oyster and long oyster were subjected to gonadal synchronization, and then the two were crossed to find that the interspecific The hybrid F1 is highly sterile, and a highly sterile hybrid F1 is obtained. It is worth noting that, in the interspecific hybridization of the genus Colossus, the hybrids of Hong Kong oyster×near river oyster, Hong Kong oyster×Portugal oyster, near river oyster×long oyster are all fully fertile, and only Hong Kong oyster and Long oyster hybrids are highly sterile ( figure 1 ).

[0018] The second step, the selection of fertile individuals: in the mature season of the hybrid F1 of Hong Kong oyster and long oyster, the fertility analysis was carried out, and the hybrid sterility showed that only 8.64% of the female individuals were fully fertile, and 5.06% o...

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Abstract

The invention discloses a fertility recovery method for oyster distant hybrid sterility. The fertility recovery method includes by a distant hybridization mode, performing synchronous gonad treatment on Hongkong oysters and pacific oysters, and performing interspecific oyster hybridization to acquire a highly-sterile filial generation F1; during maturity seasons of filial generation F1 hybrids, analyzing fertility of the hybrids and screening out fertile individuals; by a family or group reproduction mode, performing self-reproduction of the fertile individuals of the hybrids F1 to acquire a secondary filial generation F2; through molecular markers, screening out individuals relatively similar with the hybrids F1 in terms of genotype from completely-fertile hybrids F2 to acquire completely-fertile groups with characteristics of the hybrids F1 so as to realize complete hybrid fertility recovery. By the fertility recovery method, the problem of failure to continue follow-up breeding work due to oyster intersterility frequently caused during distant hybridization is solved, data and references are provided for distant backcross, multiple cross, potential estimation on hybrid offspring breeding and the like of oysters, and possibility is also provided for research and development of new species of the oysters.

Description

technical field [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine agriculture, and in particular relates to a fertility recovery method for distant hybrid sterility of oysters. Background technique [0002] Hybrid sterility (Hybridsterile) is a common phenomenon in distant hybridization, which refers to hybrids obtained between biological types with distant kinship, which cannot reproduce offspring or fecundity due to their physiological function inconsistency and reproductive system disturbance. low phenomenon. This phenomenon is mainly due to the fact that species are genetically completely different evolutionary units, and there is a reproductive isolation mechanism. Hybrid sterility mainly focuses on distant hybridization of crops and livestock (Lou Yundong, 1999). However, hybrid sterility is not absolute. For example, in fish, it can be divided into fully fertile, impeding hermaphroditism, parthenogenesis, and complete s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环肖述张扬喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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