Method for preparing synthetic hybrid seeds by utilizing cytoplasmic male sterile line of kenaf

Abstract

The invention discloses a method for preparing synthetic hybrid seeds by utilizing a cytoplasmic male sterile line of kenaf. The method comprises the following specific steps of: (1) hybridizing the cytoplasmic male sterile line of the kenaf as a female parent with a male sterile maintainer line to reproduce the cytoplasmic male sterile line, hybridizing the cytoplasmic male sterile line as the female parent with a restoring line to prepare F1-generation hybrid seeds, and hybridizing the F1-generation hybrid seeds as a male parent with the cytoplasmic male sterile line of the female parent of the F1-generation hybrid seeds as the female parent so as to construct permanent separated groups; (2) carrying out mixed planting on the plurality of permanent separated groups, and selecting relatively consistent single sterile plants into one group to obtain a plurality of basic groups; (3) carrying out open pollination on all the groups, and after multiple generations, combining plant lines without separation in all the groups to obtain a plurality of groups with balanced inheritance; (4) identifying the groups with balanced inheritance and selecting the groups conforming to the breeding object, i.e., the synthetic hybrid seeds. The method disclosed by the invention has the advantages that the prepared synthetic hybrid seeds can be utilized by multiple generations, and farmers do not need to buy the hybrid seeds for every year, so that the planting cost of the kenaf is reduced.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for preparing a comprehensive hybrid by using the cytoplasmic male sterile line of kenaf. Background technique [0002] Kenaf, also known as "kenaf", is an annual bast fiber crop of the Malvaceae Hibiscus genus. Because kenaf is cultivated for the purpose of harvesting bast fibers or stems, the heterosis rate is as high as 35% to 40%. The utilization of kenaf heterosis has long been the main goal of domestic and foreign kenaf breeding. Utilization of cytoplasmic male sterile lines of kenaf for hybrid seed production. [0003] In September 2008, the "Breeding Method of Cytoplasmic Male Sterile Line of Kenaf Wild Abortive Type" invented by Zhou Ruiyang of Guangxi University won the national invention patent (ZL200510019454.4, publication number CN100415083C). According to this method, K03A (see: Guikejianzi No. [2004] No. 152), Fu 3A (also known as F3A), P3A, 917A, 722A, 763A an...

AI Technical Summary

Problems solved by technology

Although its yield is high, farmers need to purchase hybrid seeds every year, which increases the cost of planting kenaf