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Method for detecting insecticide resistance of apolygus lucorum

A technology for the resistance of Lygus green bugs and pesticides, which is applied in the field of determination of pesticide resistance of agricultural pests, can solve the problems of shortening the time of inspection results, no improvement, cumbersome operation, etc., achieves rapid detection, convenience and reliability, reduces cumbersome steps, and solves the problem of The effect of using a large amount of insects

Inactive Publication Date: 2014-03-26
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Toxicity determination is a common method for indoor understanding of pest resistance. However, the traditional leaf drug film method and spot method are cumbersome to measure toxicity. Several concentrations must be set and repeated to ensure the reliability of the results.
The glass tube drug film method is a new method for rapid detection of pest resistance. Due to its obvious effect, it shortens the time for checking results, but it is still the same as the traditional method in terms of concentration and repetition.

Method used

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  • Method for detecting insecticide resistance of apolygus lucorum
  • Method for detecting insecticide resistance of apolygus lucorum
  • Method for detecting insecticide resistance of apolygus lucorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Obtaining of embodiment 1 diagnostic dosage

[0026] 1. Research object

[0027] The research objects of the present invention are laboratory-raised sensitive populations of Lygus aureidae that have not been exposed to any medicament.

[0028] 2. Method

[0029] The test agents were malathion, chlorpyrifos, methomyl, cyhalothrin, imidacloprid and endosulfan. The glass tube drug film method was used to determine the toxicity.

[0030] 2.1 Configuration and dilution of the mother liquor of the original drug

[0031] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C. Before the toxicity test, the original drug mother solution was diluted to various concentrations with acetone and set aside.

[0032] 2.2 Preparation of glass tube drug film

[0033] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Dr...

Embodiment 2

[0039] Embodiment 2 diagnostic dosage detects the drug resistance of Lygus chlorophylla

[0040] 1. Research object

[0041] The research object of this embodiment is the population to be tested in the field of Lygus lucidum.

[0042] 2. Method

[0043] The tested medicaments were malathion, chlorpyrifos, methomyl, cyhalothrin, imidacloprid and endosulfan. The diagnostic dosage of the present invention was used to make a glass tube drug film, and the resistance level of Lygus lucidum was tested in the field.

[0044] 2.1 Configuration and dilution of the mother liquor of the original drug

[0045] The original drug is dissolved in acetone, and the mother liquor of the original drug is diluted with the diagnostic dose obtained in Example 1 with acetone for subsequent use.

[0046] 2.2 Preparation of glass tube drug film

[0047] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing to ensure that no drug remains. Draw 220μL from the dilute...

Embodiment 3

[0051] Example 3 The traditional glass tube drug film method is to the determination of the drug resistance of the field Lygus chinensis population

[0052] 1. Research object

[0053] The object of this case study is the tested population of Lygus japonica in the field.

[0054] 2. Method

[0055] The test agents were malathion, chlorpyrifos, methomyl, cyhalothrin, imidacloprid and endosulfan. The glass tube drug film method was used to determine the toxicity.

[0056] 2.1 Configuration and dilution of the mother liquor of the original drug

[0057] Dissolve the original drug in acetone, put it in a medicine bottle after constant volume, seal it with parafilm, and store it in a refrigerator at 4°C.

[0058] Before the toxicity test, dilute the mother liquor of the original drug to 5-7 concentrations with acetone, and set aside.

[0059] 2.2 Preparation of glass tube drug film

[0060] Glass tubes used in experiments (such as figure 1 shown) must undergo rigorous washing...

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Abstract

The invention provides a method for detecting the insecticide resistance of apolygus lucorum. Under the diagnostic dose of an insecticide, a diagnostic glass tube is manufactured for rapidly detecting the resistance level of an apolygus lucorum field population to the insecticide, wherein the insecticide is malathion, chlorpyrifos, methomyl, cyhalothrin, imidacloprid or endosulfan; the diagnostic dose of the insecticide as follows: 67 ng / cm<2> of malathion, 560 ng / cm<2> of chlorpyrifos, 620 ng / cm<2> of methomyl, 3,046 ng / cm<2> of cyhalothrin, 886 ng / cm<2> of imidacloprid and 1,092 ng / cm<2> of endosulfan. The method disclosed by the invention is convenient to use and is suitable for rapidly detecting the resistance level of the apolygus lucorum in the field; results are intuitive and clear; the disadvantages that the conventional bioassay method has a large number of samples, tedious operation, time waste and the like are overcome; the method can be used for rapidly detecting the resistance level of the apolygus lucorum field population.

Description

technical field [0001] The invention relates to a measuring technique method for monitoring the drug resistance of agricultural pests, in particular to a method for detecting the drug resistance of the green Lygus bug. Background technique [0002] In recent years, with the large-scale planting of transgenic Bt cotton in my country, cotton bollworm has been effectively controlled. However, due to the reduction in the use of chemical agents in cotton fields, miridids have risen to become the main pest. At the same time, due to the adjustment of agricultural industry structure, fruit trees The planting area of ​​vegetables and vegetables has increased substantially, providing rich host plants and suitable wintering places for the mirid bug, and the occurrence and damage are becoming more and more serious. The host range of Miridae is very wide, with 147 species in 38 families, among which there are Malvaceae, Leguminosae, Compositae, Cruciferae and many kinds of fruit forest tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5085G01N2800/44
Inventor 高希武刘晓岚谭瑶许新新冯晶晶甄从爱张帅
Owner CHINA AGRI UNIV
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