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Method for preparing nano-arbutin lipidosome with supercritical carbon dioxide

A carbon dioxide, arbutin technology, applied in the direction of liposome delivery, cosmetic preparations, cosmetics, etc., can solve the problems of poor particle size monodispersity, poor liposome stability, etc., and achieve particle size and encapsulation efficiency. Easy to control, good stability, high stability effect

Inactive Publication Date: 2014-03-26
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a non-toxic arbutin carbon dioxide preparation method for defects such as toxic organic solvent residues, weak particle size monodispersity and poor liposome stability in the existing arbutin liposome preparation method. Solvent residue, good product monodispersity, good stability method of arbutin liposome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Accurately weigh 0.060g of phospholipid and 0.006g of cholesterol according to the formula (the mass ratio of phospholipid to cholesterol is 10:1), add them into 770μl absolute ethanol (the mass ratio of phospholipid to absolute ethanol is 1:10) to prepare a solution, Put it in an ultrasonic oscillator for 5 minutes, mix well and put it in the refrigerator for later use. Accurately weigh 0.012g of arbutin, dissolve it in 10ml of deionized water to prepare an arbutin solution (the mass ratio of phospholipids to arbutin is 5:1). Mix the arbutin solution and the ethanol solution of the liposome membrane material evenly, put it into a supercritical reactor, open the carbon dioxide cylinder, and the carbon dioxide gas is cooled into a liquid by a refrigerator, and then pressurized by a high-pressure pump and enters the reactor. After maintaining the pressure for 40 minutes under the conditions of 20 MPa and 50° C., release carbon dioxide gas to lower the system pressure to n...

Embodiment 2

[0024] Accurately weigh 0.060g of phospholipids and 0.012g of cholesterol according to the formula (the mass ratio of phospholipids to cholesterol is 5:1), add them into 1.53ml of absolute ethanol (the mass ratio of phospholipids to absolute ethanol is 1:20) to prepare a solution , placed in an ultrasonic oscillator for 5 minutes, mixed evenly and placed in the refrigerator for later use. Accurately weigh 0.030g of arbutin, dissolve it in 10ml of deionized water to prepare an arbutin solution (the mass ratio of phospholipids to arbutin is 2:1). Mix the arbutin solution and the ethanol solution of the liposome membrane material evenly, put it into a supercritical reactor, open the carbon dioxide cylinder, and the carbon dioxide gas is cooled into a liquid by a refrigerator, and then pressurized by a high-pressure pump and enters the reactor. After holding the pressure for 45 minutes under the conditions of 30 MPa and 35° C., release carbon dioxide gas to lower the system pressu...

Embodiment 3

[0026] Accurately weigh 0.060g of phospholipids and 0.020g of cholesterol according to the formula (the mass ratio of phospholipids to cholesterol is 3:1), add them into 380μl absolute ethanol (the mass ratio of phospholipids to absolute ethanol is 1:5) to prepare a solution, Put it in an ultrasonic oscillator for 5 minutes, mix well and put it in the refrigerator for later use. Accurately weigh 0.003g of arbutin, dissolve it in 10ml of deionized water to prepare an arbutin solution (the mass ratio of phospholipids to arbutin is 20:1). Mix the arbutin solution and the ethanol solution of the liposome membrane material evenly, put it into a supercritical reactor, open the carbon dioxide cylinder, and the carbon dioxide gas is cooled into a liquid by a refrigerator, and then pressurized by a high-pressure pump and enters the reactor. After keeping the pressure at 25 MPa and 40° C. for 30 minutes, release carbon dioxide gas to lower the system pressure to normal pressure to obtai...

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Abstract

The invention provides a method for preparing nano-arbutin lipidosome with supercritical carbon dioxide. The method comprises the following steps: weighing phospholipid and cholesterol, dissolving the phospholipid and the cholesterol in anhydrous ethyl alcohol, weighing arbutin, and dissolving the arbutin in deionized water; mixing the two liquid uniformly, adding the mixture into a supercritical reaction kettle, controlling the temperature and the pressure, and maintaining the pressure; depressurizing to obtain an arbutin lipidosome suspension. The method has the advantages that no poisonous organic solvent is used in the preparation process; environmental pollution is avoided; no poisonous resolvent residue exists in the end product; the arbutin lipidosome prepared according to the method is high in monodispersity; the encapsulating effect and stability are high; the technology is simple; the cost is low; industrialized production can be realized easily.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and in particular relates to a method for preparing nano arbutin liposomes by using supercritical carbon dioxide. technical background [0002] Arbutin (arbutin) is a natural active substance extracted from rock cabbage, black rice tree, bearberry in gerbera and other plants, and its chemical name is p-hydroxybenzene-B-D-glucopyranoside. Arbutin is a natural active substance derived from green plants. It is a skin depigmentation component that combines "green plants, safe and reliable" and "high-efficiency decolorization". It can penetrate into the skin without affecting the concentration of cell proliferation. , can effectively inhibit the activity of tyrosinase in the skin, block the formation of melanin, accelerate the decomposition and excretion of melanin by directly combining itself with tyrosinase, thereby reducing skin pigmentation, removing spots and freckles, and It does not ...

Claims

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Application Information

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IPC IPC(8): A61K8/60A61K8/14A61K31/7034A61K9/127A61Q19/02A61P17/00
Inventor 徐少洪赵斌陈超何丹农
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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