Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof

A technology of sodium-calcium exchange and gene coding, applied in the field of genetic engineering, can solve the problems of few reports on NCX gene screening, NCX gene identification, expression analysis and clustering research, etc.

Inactive Publication Date: 2014-03-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium-calcium exchangers, as important ion channel genes, have great application potential in the genetic breeding of Litopenaeus vannamei, but there are few reports on the screening of NCX genes in Litopenaeus vannamei, and the identification, expression analysis and clustering of NCX genes so far no report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof
  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof
  • Litopenaeus vannamei sodium-calcium exchanger LvNCX gene and coding protein thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Cloning and sequencing of the sodium-calcium exchanger gene LvNCX of Litopenaeus vannamei

[0030] 1. Design of primers

[0031] Taking the individual after freshwater stress for 6 hours as Tester and the individual bred at salinity 30 as Driver, a reduced cDNA library related to salinity resistance traits was constructed, and 24 colonies were randomly selected from the library for PCR identification, and the length of the inserted fragment was between 200 ~599bp. The capacity of the library was 2500 clones, and 500 clones were randomly selected for sequencing analysis. A total of 325 high-quality expressed sequence tags (ESTs) and 275 Unigenes (104 Contags and 171 Singletons) were obtained. BLASTx and BLASTn homology comparison with GenBank database, 79.1% of which are known functional genes, genes related to salinity resistance traits include Na-Ca exchange, 5' and 3' RACE primers were designed according to EST sequence, Primers were synthesized by Yingwei Jieji ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a litopenaeus vannamei sodium-calcium exchanger LvNCX gene and a coding protein thereof. A nucleotide sequence of the litopenaeus vannamei sodium-calcium exchanger LvNCX gene is as shown in SEQ ID NO.1; and an amino acid sequence of the coded sodium-calcium exchanger LvNCX protein is as shown in SEQ ID NO.2. According to the invention, sodium-calcium exchanger LvNCX gene is firstly cloned out from the litopenaeus vannamei, and the gene is proved to be capable of sustaining equilibrium of sodium and calcium ions in a cell body by virtue of experiment, so that theoretical basis is laid for further deeply analyzing biological stress-resistance ability under NCX (Sodium-Calcium Exchanger) influences and applying the NCX gene in stress-resistance research of the litopenaeus vannamei.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the LvVCX gene of the Litopenaeus vannamei sodium-calcium exchanger and its coded protein. Background technique [0002] Sodium / calcium exchanger (NCX) plays an important role in maintaining the balance of sodium and calcium ions in cells. It is a reversible membrane transporter. NCX is found on the cell membrane or the membrane of organelles , whose function is to combine 2 Na + Cells are removed, and 1 calcium ion is brought into the cell, or it can be done in the opposite direction. The function of NCX is to excrete calcium ions in a ratio of 4:1:1 (Na + / Ga2 + / K + ) is discharged in proportion, so it is Na + / K + Dependence is an important channel for maintaining the stability of intracellular ions. [0003] Litopenaeus vannamei (Litopenaeus vannamei), also known as white-footed shrimp, Penaeus vannamei, Pacific white shrimp, commonly known as ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435
CPCC07K14/43509
Inventor 王艳红任春华胡超群张吕平罗鹏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap