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Preparation method of PLGA fibrous coat used for corneal tissue transplant

A technology of tissue transplantation and PLGA, which is applied in the fields of non-woven fabrics, textiles and papermaking, medical science, etc., can solve the problems of transplantation failure and achieve good transmission performance

Inactive Publication Date: 2014-02-26
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] Full-thickness corneal transplants can temporarily restore vision in patients with corneal stem cell deficiency, but eventually the graft will fail due to a lack of essential corneal stem cells

Method used

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Examples

Experimental program
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Embodiment Construction

[0011] In order to deepen understanding of the present invention, below in conjunction with specific example, the present invention is described in further detail:

[0012] (1) Polylactide-copolyglycolide (PLGA) (60:40, molecular weight is 60kg / mol) was dissolved in dichloromethane to prepare a 50wt% solution, and then the solution was filled into four 10mL syringes, The inner diameter of the injection needle is 0.4mm; the syringe is connected to the syringe pump, and the parameters of electrospinning are: the flow rate of the polymer solution is 0.8mL / h, the spinning voltage is 20kV, and the receiving end device is covered with polytetrafluoroethylene fiber cloth Rotating drum with a rotating speed of 3500 rpm; the thickness of the finally obtained film is 80-120 μm.

[0013] (2) Dissolve the polymer in 1,1,1,3,3,3-hexafluoroisopropanol at 25°C and under gentle stirring to prepare spinning solutions with different concentrations (40wt%, 60wt%) . The polymer solution is load...

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Abstract

The invention relates to a synthetic-biodegradable PLGA fibrous coat which is prepared in an electrostatic spinning method and used for corneal tissue transplant, and in particular to a fibrous coat which is synthetic and germfree and capable of being rapidly degraded. The fibrous coat is supportive of rapid growth of separated corneal epithelial cells and has the capability that intact tissue transplant is directly placed on the coat and the corneal epithelial cells can grow outwards, and the separated cells or the cells growing outwards from the corneal transplant have good transmission performance on the coat. The fibrous coat prepared by the method can be directly used for substitute things of human amniotic membrane and has wide application prospect in the field of clinical corneal regeneration.

Description

technical field [0001] The invention relates to a preparation method of a fiber membrane, in particular to a preparation method of a biodegradable PLGA fiber membrane used for corneal tissue transplantation. Background technique [0002] At present, there are only a few specialized ophthalmic laboratories in the world that can use fibrin or human amniotic membrane to transfer corneal epithelial cells to the cornea. One approach to artificially culturing the cells is to grow the cells in situ on the corneal graft, thus avoiding the need for corneal epithelial cell expansion, at least at the laboratory level, in patients who are deficient in a single corneal stem cell. [0003] Full-thickness corneal transplants can temporarily restore vision in patients with corneal stem cell deficiency, but eventually the graft will fail due to a lack of essential corneal stem cells. In order to replace the lost stem cells, the culture and transfer of corneal epithelial cells is an optional...

Claims

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Application Information

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IPC IPC(8): D04H1/435D04H1/728A61L27/18
Inventor 韩志超许杉杉佴刚
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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