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Seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization

A technology of male sterile lines and hybrid seed production, applied in botany equipment and methods, applications, plant genetic improvement, etc., can solve the problems of not meeting the needs of cotton production and development, difficulty in ensuring the purity of seed production, and difficulty in finding labor. , to achieve the effect of simplifying seed production procedures and production links, reducing labor intensity and supervision difficulty, and avoiding uneven density

Inactive Publication Date: 2014-02-26
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the conditions for seed production are difficult, so it is becoming more and more difficult to find suitable labor
[0008] D. High production costs: Due to the continuous increase of labor prices, the cost of artificial seed production is getting higher and higher. At present, the income of seed production is only slightly higher than that of ordinary commercial cotton, which is not attractive.
[0009] In short, the artificial hybrid seed production method is the main hybrid seed production method in my country at present. This seed production method has many disadvantages such as high labor intensity, cumbersome operation procedures, high production cost, and difficulty in guaranteeing the purity of the seed production.
With the gradual improvement of our country's social living standards, labor costs have risen year after year, and artificial hybrid seed production methods have become less and less suitable for the needs of cotton production and development, and have become the main limiting factor that restricts the further development of hybrid cotton.

Method used

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  • Seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization
  • Seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization
  • Seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1 Confirmation of parental planting method

[0034] experimental method:

[0035] 1. Variety and source of parents used for seed production:

[0036] Male parent: HAF277, female parent: HAM277. Source: It is derived from the three-line material of the cotton Hackneysy cytoplasmic sterile line (CMS) introduced by the American Circle Company in 1979, and the material has been publicly distributed around the world.

[0037] 2. Sowing method and time: live broadcast, late April.

[0038] 3. The planting ratio of father and mother: 1:4.

[0039] 4. Sowing density: 3000 plants / mu.

[0040] 5. Seed production period: July 10 to August 20.

[0041] 6. The species and source of the honeybees used: Apis subspecies, commercially available.

[0042] 7. Bee release time: 8:00 am and 17:00 pm every day.

[0043] 8. The amount of bees released: 1 box per mu, 7000-10000 bees per box

[0044] 9. Farming isolation distance: more than 1 kilometer.

[0045] Sowing metho...

Embodiment 2

[0049] Embodiment 2 The confirmation of the planting row ratio of parents

[0050] Experimental method: The parents were planted alternately according to the row ratio, and four treatments were set for the planting ratio of the parents: 1:3, 1:4, 1:5, and 1:6 for comparison, and the others were the same as in Example 1. The results are shown in Table 2.

[0051] Table 2 The effect of parental ratio on cotton cytoplasmic sterile line using insect hybrid seed production

[0052]

[0053] Through small-scale field experiments on bee pollination in Nanyang and Anyang, the effects of insect pollination and seed production under different planting ratios of parents were systematically compared and analyzed, and the conclusions were drawn as follows: the parents were planted in a ratio of 1:3 The number of bolls per plant, the yield of seed cotton and the yield of seeds were the highest in seed production, and the boll number per plant, yield of seed cotton and seed yield of seed...

Embodiment 3

[0054] Confirmation of Example 3 Pollinating Insects

[0055] Experimental method: Two treatments of Italian bee and bumblebee were set up for pollination insects to compare. The sources are all purchased from the market, and the parents are planted alternately at a row ratio of 1:4.

[0056] The experiment compared the pollination effect of honeybee and bumblebee species, and compared it with the effect of artificial pollination. The results showed that the pollination effect of bees was relatively ideal, which was similar to the yield of artificial pollination seed production in the same period, which was equivalent to the artificial pollination system. About 80% of the seed production. It is difficult to raise bumblebees, the cost is high, and the source of bees is difficult to find, while honey bees are more popular, easy to raise, and low in cost. The Italian subspecies of bees is suitable for breeding in plains (large honey sources), so it is a more suitable pollinator. ...

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Abstract

A disclosed seed production method for cotton cytoplasmic male sterile line by utilizing insect hybridization is characterized by comprising: under the isolation condition, taking cotton cytoplasmic male sterilty male sterile line as a female parent, taking cotton restorer line as a male parent, planting the female parent and the male parent according a line number ratio of 1:4, and taking honeybee as pollination insect to perform cotton hybridization for seed production. According to the method, a box of honeybee can take the place of the pollination workload of one person, the seed production output at a normal year accounts for about 80% of the output of manual seed production, and thus labor is saved, labor intensity is reduced, seed production benefit is improved, and the purity and the quality of the hybridization seeds are guaranteed because of no selfing.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for producing cotton cytoplasmic male sterile lines by hybridizing insects. Background technique [0002] The research and utilization of heterosis can significantly increase the yield, quality and resistance of cotton. Under normal circumstances, hybrid varieties can increase production by more than 10% compared with conventional varieties, and some can reach 30-50%. my country's cotton heterosis research began in the 1930s, but it was not applied to production until the 1980s. However, limited by the research level and production conditions at that time, the production can only use the second generation of hybrids with weak advantages. host. At the end of the 1990s, with the advent of the insect-resistant hybrid Zhong Cotton Institute 29 and the establishment of an efficient artificial hybrid seed production system, the scale and intensification of artificial hybrid seed prod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 郭立平邢朝柱吴建勇戚廷香王海林唐会妮
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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