A test strip and method for detecting neomycin

A technology of neomycin and test strips, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems that are not suitable for on-site monitoring and screening of a large number of samples, cumbersome operation process, complex instruments and equipment, etc., and achieve faster chromatography Speed, ease of operation, and high-sensitivity effects

Active Publication Date: 2016-04-20
BEIJING KWINBON BIOTECH
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to complex instruments and equipment and cumbersome operation process, it is not suitable for on-site monitoring and screening of a large number of samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A test strip and method for detecting neomycin
  • A test strip and method for detecting neomycin
  • A test strip and method for detecting neomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 detects the composition of the test strip of neomycin

[0042] 1. Test paper ( figure 1 )

[0043] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0044] The sample absorbent pad 1, the reaction film 2, the water absorbent pad 3 and the protective film 7 are pasted on the base plate 6 in sequence, the end of the sample absorbent pad is connected with the reaction film, the end of the reaction film is connected with the water absorbent pad, the sample absorbent pad The beginning is aligned with the beginning of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0045] The end of the sample absorption pad of the test paper is pasted with a protective film, and the protective film 7 covers the detection end on the sample absorption pad, and the word MAX is printed on the detection end protective film ( figure 2 );

[004...

Embodiment 2 Embodiment 1

[0051]The preparation method of test strip described in embodiment 2 embodiment 1

[0052] 1. Preparation of test strips

[0053] The preparation method of test strip mainly comprises the following steps:

[0054] 1) Prepare freeze-dried microwell reagents with neomycin monoclonal antibody-colloidal gold markers;

[0055] 2) Prepare a reaction membrane with a detection area coated with neomycin hapten-carrier protein conjugate and a quality control area coated with goat anti-mouse anti-antibody;

[0056] 3) Assemble the reaction film prepared in 2) with the sample absorbent pad, water absorbent pad, protective film, and bottom plate to form a test paper;

[0057] 4) Assemble the freeze-dried microwell reagent and test paper prepared in 1) and 3) with neomycin monoclonal antibody-colloidal gold marker into a test strip.

[0058] The following step-by-step detailed description:

[0059] (1) Preparation of each component

[0060] 1. Synthesis and identification of neomycin h...

Embodiment 3

[0102] Application of embodiment 3 test strips

[0103] The test strip of the invention can detect the neomycin residue in milk. The general detection method is as follows:

[0104] 1. Detection method

[0105]Take out the required number of microwell reagents and test papers from the original package, and mark them; use a micropipette to pipette 200 μl of the milk sample to be tested into the microwell, suck slowly and fully mix with the reagent in the microwell, After incubating at room temperature (20°C-25°C) for 5 minutes; insert the end of the printed "MAX" line down into the microwell to fully immerse it in the solution; after incubating at room temperature (20°C-25°C) for 5 minutes, take out the test paper and judge result.

[0106] 2. Analysis of test results

[0107] Positive: When the quality control area (C) shows a band and the detection area (T) does not develop color, it is judged as positive, and it is indicated by "+", such as Figure 4 a; Negative: When bo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a test strip and method for detecting neomycin. The test strip comprises a micro-porous reagent and test paper, wherein a colloidal-gold-labeled neomycin monoclonal antibody is subjected to freeze-drying in the micro-porous reagent; the test paper is formed by sequentially connecting a sample absorption pad, a reactive film, a water absorption pad, a protective film and a bottom plate; a detection area and a quality control area are formed in the reactive film; a neomycin hapten-carrier protein conjugate is coated in the detection area; an anti-antibody is coated in the quality control area. The method for detecting the neomycin by using the test strip is simple, rapid, intuitive, accurate, wide in application range, low in cost and easy to popularize and use.

Description

technical field [0001] The invention relates to a test strip and a method for detecting neomycin, in particular to a colloidal gold test strip for detecting neomycin in milk samples. Background technique [0002] Neomycin (Neomycin, NEO) is an aminoglycoside antibiotic, which is composed of neomycin and neomycin. It has a broad antibacterial spectrum and has good inhibitory effects on Gram-negative bacteria, Gram-positive bacteria, Mycobacterium tuberculosis, and acid-fast bacteria. As neomycin is more and more widely used in the breeding industry at home and abroad, its residue problem in animal food is also becoming more and more prominent, and it has potential ototoxicity and nephrotoxicity to humans and animals, causing serious harm to human health. posed a great threat. At present, the detection methods of neomycin residue mainly include microbiological method, high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and immunological assay. Due ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/531
CPCG01N33/531G01N33/558G01N33/577
Inventor 冯才伟聂雯莹何方洋付军权罗晓琴朱亮亮余厚美张荃
Owner BEIJING KWINBON BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap