Wheat CBL-CIPK (CBL-interacting protein kinase) stress tolerance regulatory factor as well as encoding gene and application thereof

Abstract

The invention relates to a wheat CBL-CIPK (CBL-interacting protein kinase) stress tolerance regulatory factor as well as an encoding gene and an application thereof. According to the wheat CBL-CIPK stress tolerance regulatory factor, protein kinase genes which are consciously induced by salt and drought when being expressed in roots and leaves are selected according to expression profile chip information of salt and drought stress of wheat, and a cDNA (complementary deoxyribonucleic acid) sequence with a whole-length encoding area is cloned, wherein the nucleotide sequence is as shown in SEQ ID NO.1. Analysis shows that the gene can encode CBL-CIPK serine / threonine protein kinase, the amino acid sequence is as shown in SEQ ID NO.2, and is named as TaCIPK33. Transgenic function analysis shows that the gene has the functions of improving plant salt tolerance and drought resistance. Basis is provided for further understanding molecular mechanisms of wheat in responding to drought and saltstress, and meanwhile the gene can be applied to genetic improvement of novel stress tolerance germplasms of crops such as wheat.

Description

technical field [0001] The invention relates to a wheat CBL-CIPK stress-resistance regulating factor and its encoding gene and application, and belongs to the technical fields of molecular biology technology and genetic engineering. Background technique [0002] Wheat is the staple food for about 35% of the world's population. Drought, salinization and other adversities are important environmental factors that limit the yield and quality of wheat, and can easily lead to infection by diseases and insect pests, resulting in large economic losses. However, wheat cannot escape adverse environments. Therefore, cloning drought-resistant and salt-tolerant genes, studying the molecular mechanisms of drought-resistant and salt-tolerant genes, and cultivating new drought-resistant and salt-tolerant varieties have great theoretical and practical significance. [0003] Serine / threonine protein kinases are involved in plant responses to different stresses and can play a role in improvin...

AI Technical Summary

Problems solved by technology

Drought, salinization and other adversities are important environmental factors that limit the yield and quality of wheat, and can easily lead to infection by diseases and insect pests, resulting in large economic losses

However, wheat cannot escape the adverse environment. Therefore, cloning drought-resistant and salt-tolerant genes, studying the molecular mechanism of drought-resistant and salt-tolerant genes, and cultivating new drought-resistant and salt-tolerant varieties have great theoretical and practical significance.

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