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Liproteins, preparation method and application of liproteins in preparing medicament for preventing and treating tumor diseases

A technology of lamprey protein, applied in the field of application of lamprey protein, preparation and preparation of drugs for preventing and treating tumor diseases

Active Publication Date: 2014-02-05
LIAONING NORMAL UNIVERSITY
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Problems solved by technology

However, so far, there is no information about a protein with a lectin domain (Jacalin_like domain) and a pore-forming toxin domain (Pore-forming toxin_like domain) isolated from the culture medium of Lamprey Lee's body cells and the application of this protein Related reports on the preparation of drugs for the prevention and treatment of tumor diseases

Method used

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  • Liproteins, preparation method and application of liproteins in preparing medicament for preventing and treating tumor diseases
  • Liproteins, preparation method and application of liproteins in preparing medicament for preventing and treating tumor diseases
  • Liproteins, preparation method and application of liproteins in preparing medicament for preventing and treating tumor diseases

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Embodiment Construction

[0025] 1. Preparation of Total Proteins Secreted by Lamprey Listeri Bodies:

[0026] a. Dissect live adult lampreys to obtain fresh Leigh bodies;

[0027] b. PBS was washed to remove the residual blood in the Lew body tissue;

[0028] c. Cut the Lee bodies into 1 mm with scissors 3 Digest the small pieces in trypsin overnight at 4°C;

[0029] d. The digested cells were collected the next day and washed twice with PBS;

[0030] e. Transfer to serum-free 1640 culture medium, wait for cells to secrete soluble protein;

[0031] f. After 72 hours, the culture solution of Lamprey Lee's body cells was collected and freeze-dried to make a dry powder.

[0032] 2. Isolation and Purification of Lamprey Li Proteins

[0033] a. Dissolve the dry total protein powder with 200 ml of distilled water to obtain a total protein solution containing plum protein;

[0034] b. Add phenylmethylsulfonyl fluoride (PMSF) at a final concentration of 2 mmol / L to the total protein solution t...

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Abstract

The invention discloses liproteins, a preparation method and an application of liproteins in preparing a medicament for preventing and treating tumor diseases. The liproteins are pure natural proteins which are separated and purified from a cell culture fluid of lamprey corpuscle, is named Liproteins, has a Jacalin_like domain and a Pore-formingtoxin_like domain, and can be synthesized through expression of a heterologous expression system to form recombinant protein of liproteins. The liproteins have a specific killing function of tumor cells, does not destroy normal cells, and does not have side effect to human bodies.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, in particular to a lamprey plum protein capable of specifically killing tumor cells and having a lectin domain (Jacalin_like domain) and a pore-forming toxin domain (Pore-forming toxin_like domain), and preparation thereof The method and its application in the preparation of drugs for the prevention and treatment of tumor diseases. Background technique [0002] The World Health Organization predicts that by 2030, the global annual cancer death toll may double on the existing basis, and by then 17 million people will die of cancer every year. Although people's understanding of tumor occurrence and deterioration has been continuously improved, and a large number of literature reports have made some major breakthroughs in the field of tumor treatment, however, there is still no safe and effective drug for the prevention and treatment of tumor diseases, so tumors are often used. Described as "incur...

Claims

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Application Information

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IPC IPC(8): C07K14/46C12P21/06C07K1/34A61K38/17A61P35/00
CPCA61K38/1706C07K14/461
Inventor 李庆伟逄越于涛
Owner LIAONING NORMAL UNIVERSITY
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