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Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid

A technology of ethylenediaminetetraacetic acid and lead-contaminated soil, applied in the direction of restoration of polluted soil, etc., to achieve the effects of promoting plant growth, promoting extraction and enrichment, and increasing biomass

Inactive Publication Date: 2014-01-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no relevant gibberellin (GA 3 ) alone or in combination with ethylenediaminetetraacetic acid (ETDA) for enhanced phytoremediation of lead-contaminated soils

Method used

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  • Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid
  • Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid
  • Method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid

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Experimental program
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Effect test

Embodiment 1

[0017] The experimental site was set in the greenhouse of the School of Environment, Zhejiang Gongshang University. The soil for potting experiments was collected from a farmland in the outskirts of Hangzhou, and the soil type was clay. The lead concentration of the soil used was 250 mg / kg. The study found that compared with one-time addition, the amount of lead in the soil was extracted and transported by plants when adding EDTA in divided doses (document 4: Hadi, F., Bano, A., Fuller, M.P. 2010. The improved phytoextraction of lead(Pb) and the growth of maize(Zea mays L.): the role of plant growth regulators(GA 3 and IAA) and EDTA alone and in combinations[J]. Chemosphere80,457-462.). Moreover, when the EDTA concentration is 2.5mmol / kg, buckwheat and sunflower have the strongest ability to absorb and transport lead, so the EDTA concentration is set at 2.5mmol / kg in this experiment (Reference 5: Chen, Y.H., Li, X.D., et al., 2004. Leaching and uptake of heavy metals by ten ...

Embodiment 2

[0032] The experimental site was set in the greenhouse of the School of Environment, Zhejiang Gongshang University. The soil for potting experiments was collected from a farmland in the outskirts of Hangzhou, and the soil type was clay. The lead concentration of the soil used was 500 mg / kg. Put the test soil into pots after passing through a 5mm sieve, and fill each pot with 18.5cm (diameter)×21cm (height) with 2kg (dry weight) of soil, and put a tray under the pot. Pb(NO 3 ) 2 Add the soil in the form of aqueous solution, and mix well, the measured lead concentration in the soil is 500mg / kg, add water to 75% of the field water capacity, and use it for the test after being stable in the greenhouse for 30 days. A total of 8 experimental groups were set up in the experiment, namely: without adding EDTA and GA 3 (C), add 2.5mmol / kg EDTA (T1), leaf spray 1μM GA 3 (T2), leaves sprayed with 10 μM GA 3 (T3), leaves sprayed with 100 μM GA 3 (T4), 2.5mmol / kg EDTA+ leaf spray 1μM ...

Embodiment 3

[0047] The experimental site was set in the greenhouse of the School of Environment, Zhejiang Gongshang University. The soil for potting experiments was collected from a farmland in the outskirts of Hangzhou, and the soil type was clay. The lead concentration of the soil used was 750 mg / kg. Put the test soil into pots after passing through a 5mm sieve. Each pot is 18.5cm (diameter) × 21cm (height) and filled with 2kg (dry weight) of soil, and a tray is placed under the pot. Pb in the tested soil was expressed as Pb(NO 3 ) 2 Add the soil in the form of an aqueous solution and mix it thoroughly. The measured lead concentration in the soil is 750mg / kg, then add water to 75% of the field water holding capacity, and use it for the test after being stable in the greenhouse for 30 days. A total of 8 experimental groups were set up in the experiment, namely: without adding EDTA and GA 3 (C), add 2.5mmol / kg EDTA (T1), leaf spray 1μM GA 3 (T2), leaves sprayed with 10 μM GA 3 (T3), ...

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Abstract

The invention relates to a method for strengthening remediation of lead contaminated soil by combining gibberellin and ethylenediamine tetraacetic acid. Ryegrass is planted in the lead contaminated soil, gibberellin (GA3) is added in a foliar spray form, and the ethylenediamine tetraacetic acid (EDTA) is directly added into the soil, therefore, the purpose of strengthening remediation of the lead contaminated soil by the plants is achieved. The GA3 at the concentration of 1mu M or 10mu M can obviously relief toxicity for the plants caused by Pb and / or EDTA, and the effect of strengthening remediation of the EDTA can be significantly improved. However, 100mu M GA3 inhibits the growth of the plants, and significantly reduces the concentration of lead in the plants. The method provided by the invention is used for carrying out in situ remediation, has the advantages of low cost, simple process, environmental friendliness and the like, has a good remediation effect on lead contaminated soil, and is suitable for remediation of lead contaminated soil.

Description

technical field [0001] The present invention relates to the technique of intensified remediation of heavy metal lead-contaminated soil, and relates to a method for intensified remediation of lead-contaminated soil by combining gibberellin and ethylenediaminetetraacetic acid, specifically a method using gibberellin (GA 3 ) and ethylenediaminetetraacetic acid (ETDA) combined to strengthen the remediation method of lead-contaminated soil. Background technique [0002] Phytoremediation technology has the advantages of simple operation, economy, environmental friendliness and easy acceptance by people, and has become a research hotspot in the field of soil heavy metal pollution remediation. Due to the low phytoavailability of lead in soil, the slow growth of heavy metal hyperaccumulating plants and small organisms, and the low concentration of heavy metals in plants with large biomass, this severely limits the application of phytoremediation technology in actual contaminated soil...

Claims

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Application Information

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IPC IPC(8): B09C1/08
Inventor 何闪英吴秋玲张华张熹王雷
Owner ZHEJIANG GONGSHANG UNIVERSITY
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