Silkworm pupa polypeptide skin-smoothing mask and preparation method thereof
A technology of silkworm chrysalis protein polypeptide and silkworm chrysalis, which is applied in the field of cosmetics and skin care products to achieve the effects of delaying skin aging, increasing added value, and low price
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Embodiment 1
[0040] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide
[0041] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 55°C, the pH value is 7.0, the enzymatic hydrolysis time is 3h, and the enzymatic hydrolysis is completed at 92°C Inactivate the enzyme for 13 minutes;
[0042] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0043] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...
Embodiment 2
[0069] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide
[0070] (1) Prepare a silkworm chrysalis protein solution with a concentration of 10% with deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymatic hydrolysis temperature is 50°C, the pH value is 7.5, the enzymatic hydrolysis time is 4h, and the enzymatic hydrolysis is completed at 95°C Inactivate the enzyme for 10 minutes;
[0071] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.5, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 50°C, and the enzyme is extinguished at 95°C after the enzymatic hydrolysis is completed. Enzyme 10min;
[0072] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, cle...
Embodiment 3
[0080] Step 1: Two-step enzymatic hydrolysis to prepare silkworm chrysalis protein polypeptide
[0081] (1) Prepare silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, enzyme amount 60000IU / g silkworm chrysalis protein, 3%, enzymolysis temperature 45℃, pH value 6.5, enzymolysis time 2h, enzymolysis is completed Then inactivate the enzyme at 90°C for 15 minutes;
[0082] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 10.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 4 hours, the enzymatic hydrolysis temperature is 45°C, and the enzymatic hydrolysis is extinguished at 90°C Enzyme 15min;
[0083] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfi...
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