Yoghurt with sea cucumber polypeptide and processing method for yoghurt
A sea cucumber polypeptide and yogurt technology, which is applied in the directions of milk preparations, dairy products, applications, etc., can solve the problems of adding peptides from sea cucumber viscera, which are not seen, and achieve the effects of reducing environmental pollution and resource waste, being easy to absorb, and improving economic benefits.
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Embodiment 1
[0029] Remove the sediment from the viscera of sea cucumbers, clean them, drain the water, take 10kg, homogenate with 40kg of distilled water, mix well, adjust the pH value to 5.8, and at a temperature of 35°C, the enzyme activity ratio is 1:1 Add bromelain and trypsin, the total amount of enzyme added is 6000 enzyme activity units, after enzymolysis for 2 hours, heat the enzymolysis solution to 90°C and keep it for 10 minutes to inactivate the enzyme; the enzymolysis solution after inactivation treatment is cooled to room temperature Finally, centrifuge at 4000r / min for 20 minutes to remove macromolecular proteins and insoluble impurities; take the supernatant and add a certain amount of 80-mesh powdered activated carbon to make the final percentage of 1.5%. Decolorize and deodorize for 20 minutes under certain conditions, filter and collect the filtrate, and then perform ultrafiltration through an ultrafiltration membrane filtration device with a molecular weight cut-off of 5...
Embodiment 2
[0031] Remove the sediment from the sea cucumber viscera, clean it, drain the water, take 20kg, homogenize it with 100kg distilled water, mix well, adjust the pH value to 6.3, and at a temperature of 40°C, the enzyme activity ratio is 1:3 Add bromelain and trypsin, the total amount of enzyme added is 7000 units of enzyme activity. After 3 hours of enzymolysis, heat the enzymolysis solution to 100°C and keep it for 10 minutes to inactivate the enzyme; Centrifuge for 30 minutes to remove macromolecular proteins and insoluble impurities; take the supernatant, add a certain amount of 80-mesh powdered activated carbon to make the final percentage of 1.0%, and decolorize and decolorize under the conditions of 35 ° C and pH 5.0 Fishy for 20 minutes, filter and collect the filtrate, then perform ultrafiltration through an ultrafiltration membrane filter device with a molecular weight cut-off of 5000 Daltons, and filter to obtain 115 kg of enzymatic hydrolyzate of sea cucumber viscera w...
Embodiment 3
[0033] Remove the sediment from the viscera of sea cucumbers, clean them, drain the water, take 10kg, homogenate with 80kg of distilled water, mix well, adjust the pH value to 5.8, and at a temperature of 45°C, the enzyme activity ratio is 1:2 Add bromelain and trypsin, the total amount of enzyme added is 8000 units of enzyme activity. After 3 hours of enzymolysis, heat the enzymolysis solution to 95°C and keep it for 20 minutes to inactivate the enzyme; Centrifuge for 20 minutes to remove macromolecular proteins and insoluble impurities; take the supernatant, add a certain amount of 80-mesh powdered activated carbon to make the final percentage of 1.5%, and decolorize and decolorize at 30°C and pH5.0 Fishy for 10 minutes, filter and collect the filtrate, then perform ultrafiltration through an ultrafiltration membrane filtration device with a molecular weight cut-off of 5000 Daltons, and filter to obtain 80 kg of enzymatic hydrolyzate of sea cucumber viscera with a molecular w...
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