Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunoaffinity adsorbent enriched with chloramphenicol and/or chloramphenicol base and its preparation method and application

A technology of chloramphenicol alkali and chloramphenicol, applied in chemical instruments and methods, preparation of test samples, and other chemical processes, can solve problems such as chloramphenicol residues and great harm to human health, and achieve the elimination of impurities Effects of interference, reducing sample passing time, and improving detection accuracy and reliability

Active Publication Date: 2015-10-28
GUANGXI GRAIN & OIL SCI RES INST CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the low price and obvious effect of chloramphenicol, there are still illegal use and repeated prohibitions, resulting in serious residues of chloramphenicol in food, which is extremely harmful to human health

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunoaffinity adsorbent enriched with chloramphenicol and/or chloramphenicol base and its preparation method and application
  • Immunoaffinity adsorbent enriched with chloramphenicol and/or chloramphenicol base and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, prepare the immunoaffinity adsorbent enriched in chloramphenicol and / or chloramphenicol base

[0041] This example shows the preparation of a conjugate obtained by coupling a polyclonal antibody to chloramphenicol and a solid-phase carrier oxidized by periodic acid as an immunoaffinity adsorbent for enriching chloramphenicol and / or chloramphenicol base method. The method includes:

[0042] 1. Preparation of chloramphenicol base-specific polyclonal antibody

[0043] 1.1 Preparation of chloramphenicol hapten (iodoacetyl chloramphenicol base)

[0044] 1.1.1 Dissolve 50mg iodoacetic acid (0.269mmol) and 80mg N-hydroxysuccinimide (NHS) (0.695mmol) in 500uL tetrahydrofuran (THF) to obtain solution 1; Imine (DCC) (0.582 mmol) was dissolved in 500 uL THF to obtain solution 2. Mix solution 1 and solution 2, carry out condensation reaction in the dark at room temperature (25°C) for 30 minutes on a shaker with a rotating speed of 100 rpm and a rotation radius of ...

Embodiment 2

[0068] Example 2, the immunoaffinity chromatographic column enriched with chloramphenicol and / or chloramphenicol base and its characteristic detection

[0069] 1. Preparation of an immunoaffinity chromatographic column enriched with chloramphenicol and / or chloramphenicol base

[0070] Take 25 μL of the immunoaffinity adsorbent enriched in chloramphenicol and / or chloramphenicol base prepared in Example 1 as a filler and put it into an affinity chromatography plastic column with a capacity of 1 mL. 50μm sieve plate, after the sieve plate is just in contact with the filler (do not press the filler tightly), get 25μL of immunoaffinity chromatography column enriched with chloramphenicol and / or chloramphenicol base-miniature high-efficiency enrichment of chloramphenicol and / or chloramphenicol Mycin base immunoaffinity chromatography column.

[0071] The storage conditions of the immunoaffinity chromatographic column enriched with chloramphenicol and / or chloramphenicol base: use pho...

Embodiment 3

[0086] Example 3, the immunoaffinity chromatographic column enriched with chloramphenicol and / or chloramphenicol base is used in conjunction with a colloidal gold test strip to test the sample to be tested

[0087] 1. Qualitative determination

[0088] Prepare 10 25 μL immunoaffinity chromatographic columns enriched with chloramphenicol and / or chloramphenicol base, each prepared as follows: take 25 μL of the enriched chloramphenicol and / or chloramphenicol base prepared in Example 1 The immunoaffinity adsorbent was used as packing material in an affinity chromatography plastic column with a capacity of 1 mL, and a sieve plate with a pore size of 50 μm was added to both ends of the packing material, and 25 μL of enriched chloramphenicol and / or Immunoaffinity column for chloramphenicol base.

[0089] Randomly sample four 25 μL immunoaffinity columns enriched with chloramphenicol and / or chloramphenicol base, wash the column with 3 mL of PBSt, then wash the column with 5 mL of PBS...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
adsorption capacityaaaaaaaaaa
Sensitivityaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The present invention discloses an immunoaffinity absorbent for enriching chloramphenicol and / or chloramphenicol base, a preparation method and an application thereof. The immunoaffinity absorbent is a conjugate obtained through conjugating chloramphenicol polyclonal antibody and a periodic acid-oxidized solid phase carrier, wherein the chloramphenicol polyclonal antibody is prepared according to the following steps: adopting an iodacetyl chloramphenicol base and carrier protein conjugate as immunogen to immunize non-human mammal to obtain antiserum, and purifying the antiserum to obtain the chloramphenicol polyclonal antibody. The immunoaffinity absorbent for enriching the chloramphenicol and the affinity chromatography column thereof have the following advantages that: chloramphenicol concentration in a sample requiring detection can be concentrated, a detection lower limitation is increased, impurity interference can be eliminated, detection accuracy and reliability can be increased, and a sample purifying time through the column is reduced so as to achieve rapid detection.

Description

technical field [0001] The invention relates to an immunoaffinity adsorbent enriched with chloramphenicol and / or chloramphenicol base, a preparation method and application thereof. Background technique [0002] Chloramphenicol (CAP) is a broad-spectrum antibiotic. It has been used in clinical medicine and veterinary medicine to treat various infectious diseases of humans and animals. However, chloramphenicol has serious side effects on the hematopoietic system and has been banned from clinical medicine and animal production. Our country stipulates that it shall not be detected in the edible tissues of animals. However, due to the low price and obvious effect of chloramphenicol, there are still illegal use and repeated prohibitions, resulting in serious residues of chloramphenicol in food, which is extremely harmful to human health. Immunoaffinity chromatographic column uses the principle of antigen-antibody specific recognition to quantitatively detect chloramphenicol res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/281B01J20/30G01N1/40
Inventor 刘运龙谢军李炜李川山周晓韦素梅谢体三萧浩潘艳坤徐德林林丰
Owner GUANGXI GRAIN & OIL SCI RES INST CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com