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Rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells

A technology of suspension cells and callus, applied in biochemical equipment and methods, plant cells, tissue culture, etc., can solve the problems of yield and quality decline, lack of natural resources of Astragalus membranaceus, etc., achieve less pollution, facilitate large-scale production operations, fast growth effect

Inactive Publication Date: 2013-12-18
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the dosage of Astragalus membranaceus is increasing, but the natural resources of Astragalus membranaceus are poor. During the cultivation process, due to the influence of diseases and insect pests, the yield and quality have declined. Therefore, the method of genetic technology is used to develop and produce medicinal components of Astragalus membranaceus. Plant resource development, a new way to reduce land occupation

Method used

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  • Rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Take the young leaves and shoots of Astragalus membranaceus in spring, soak in washing powder for 4 minutes, rinse with running water for 50 minutes, disinfect with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, inoculate into MS medium for callus induction, Additional hormone 1mg / LNAA, 3mg / L2, 4-D, sucrose 30g / L, agar 7g / L, pH=8, temperature 25°C, humidity 50%-70%, light intensity 2000LX; light period 14h dark period 10h ; Take the induced tender green and loose callus, inoculate it into a 100 mL Erlenmeyer flask containing 40 mL of MS liquid medium, the medium formula is MS+0.5mg / LNAA+1mg / LIBA, and place it on a rotary shaker after inoculation Vibration culture, the shaker speed is 100-120 r / min, the temperature is 25°C, the initial liquid culture callus grows vigorously, shake the culture medium and let it stand for a while, use the suspended cell mass with moderate particles for subculture, replace with 200 mL Erlenmeyer flask was cultured wit...

Embodiment 2

[0020] Take the young leaves and shoots of Astragalus membranaceus in spring, soak in washing powder for 4 minutes, rinse with running water for 50 minutes, disinfect with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, inoculate into MS medium for callus induction, Additional hormone 0. 1mg / LNAA, 3mg / L2, 4-D, sucrose 30g / L, agar 7g / L, pH=8, temperature 25°C, humidity 50%-70%, light intensity 2000LX; light period 14 h dark period of 10 hours; take the induced tender green and loose callus, inoculate it into a 100 mL Erlenmeyer flask filled with 40 mL of MS liquid medium, the medium formula is MS+0.5mg / LNAA+2mg / LIBA, and place it in a rotary Vibrating culture on a shaking table, the speed of the shaking table is 100-120 r / min, and the temperature is 25°C. The initial liquid culture callus grows vigorously. After shaking the culture medium, let it stand for a while, and use the suspended cell mass with moderate particles for subculture. Replace with 60 mL l...

Embodiment 3

[0022] Take the young leaves and shoots of Astragalus membranaceus in spring, soak in washing powder for 4 minutes, rinse with running water for 50 minutes, disinfect with sodium hypochlorite for 12 minutes, rinse with sterile water 5 times, inoculate into MS medium for callus induction, Additional hormone 1mg / LNAA, 3mg / L2, 4-D, sucrose 30g / L, agar 7g / L, pH=8, temperature 25°C, humidity 50%-70%, light intensity 2000LX; light period 14h dark period 10h ; Take the induced tender green and loose callus, inoculate it into a 100 mL Erlenmeyer flask filled with 40 mL of MS liquid medium, the medium formula is MS+1mg / LNAA+1mg / LIBA, and place it on a rotary shaker after inoculation For culture, the rotation speed of the shaker is 100-120 r / min, and the temperature is 25°C. The initial liquid culture callus grows vigorously. After shaking the culture medium, let it sit for a while, and carry out subculture with a suspended cell mass with moderate particles, and replace it with 200 Cult...

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Abstract

The invention provides a rapid propagation method of astragalus membranaceus calluses and astragalus membranaceus suspension cells. The rapid propagation method comprises the steps of obtaining a sterile explant, inducing calluses, culturing the suspension cells, regulating and controlling the activity of an enzyme in the suspension cells by using rare earth and the content of flavone saponins, and the like. The astragalus membranaceus suspension cells prepared by the method provided by the invention have high content of astragalus membranaceus saponins. The method provided by the invention realizes goal-directed regulation and control on the astragalus membranaceus suspension cells, thus a large amount of pharmaceutical raw materials can be obtained in short term and the production cost is effectively reduced.

Description

technical field [0001] The invention relates to the cultivation of astragalus suspension cells under tissue culture conditions, in particular to the regulation of astragalus saponins in the suspension cells by rare earth cerium nitrate. Background technique [0002] Astragalus, also known as Mianqi, Mianhuangqi, is a leguminous plant, Astragalus membranaceus. It is a national third-level protected plant and is an endangered species. It is mainly distributed in northern China. It is a relatively rare Chinese medicinal material. Its roots are used as medicine. With a long history, it has the effects of invigorating qi and solidifying the exterior, diuresis and detoxification, astringing sores and promoting granulation, and nourishing qi and invigorating the middle. At present, the dosage of Astragalus membranaceus is increasing, but the natural resources of Astragalus membranaceus are poor. During the cultivation process, due to the influence of diseases and insect pests, the ...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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