Method for promoting zoysia japonica seed germination
A technology for zoysia and seeds, applied in the directions of seed and rhizome treatment, application, agriculture, etc., can solve the problems of shortening germination time, low germination rate and germination consistency, and achieves good sterilization effect, improves germination consistency, and disinfection effect. outstanding effect
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Embodiment 1
[0048] 1) On August 20, 2011, the selected Zoysia seeds were soaked with 20% sodium hydroxide at 36° C. for 40 minutes; then rinsed with distilled water for 5 times to clean the residual alkali solution on the surface of the seeds.
[0049] 2) Spread the seeds on kraft paper, place them in a blast oven, and bake them at 35° C. for 1.5 hours for drying.
[0050] 3) Soak the dried grass seeds in 70% alcohol for 30 seconds, take them out and rinse them with sterile water for 3 to 5 times to wash off the alcohol on the surface of the seeds.
[0051] 4) Transfer the seeds soaked in alcohol to 25% sodium hypochlorite solution, add 4 drops of Tween-20 (volume equivalent to 1.5% of sodium hypochlorite solution) in the sodium hypochlorite solution, soak for 60 minutes, and rinse with sterile water after soaking Wash 5-6 times.
[0052] 5) Lay double-layer filter paper on the bottom of the watch glass, moisten it with sterile water, and be careful not to generate air bubbles. Select 10...
Embodiment 2
[0055] 1) On August 20, 2011, the selected Zoysia seeds were soaked with 10% sodium hydroxide at 40° C. for 30 minutes; then rinsed with distilled water for 3 to 4 times to clean the residual alkali solution on the surface of the seeds.
[0056] 2) Spread the seeds on kraft paper, place them in a blast oven, and bake them at 30° C. for 1 hour to perform drying treatment.
[0057] 3) Soak the dried grass seeds in 60% alcohol for 40 seconds, take them out and rinse them with sterile water for 2 to 3 times to wash off the alcohol on the surface of the seeds.
[0058] 4) Transfer the seeds soaked in alcohol to 20% sodium hypochlorite solution, add 6 drops of Tween-20 (volume equivalent to 2% of sodium hypochlorite solution) in the sodium hypochlorite solution, soak for 40 minutes, and rinse with sterile water after soaking Wash 5-6 times.
[0059] 5) Lay double-layer filter paper on the bottom of the watch glass, moisten it with sterile water, and be careful not to generate air b...
Embodiment 3
[0062] 1) On August 20, 2011, the selected Zoysia seeds were soaked in 30% sodium hydroxide at 30° C. for 60 minutes; then rinsed with distilled water for 3 times to clean the residual alkali solution on the surface of the seeds.
[0063] 2) Spread the seeds on the kraft paper, place them in a blast oven, and bake them at 40° C. for 2 hours for drying treatment.
[0064] 3) Soak the dried grass seeds in 80% alcohol for 20 seconds, take them out and rinse them with sterile water for 3 to 4 times to wash off the alcohol on the surface of the seeds.
[0065] 4) Transfer the seeds soaked in alcohol to 30% sodium hypochlorite solution, add 2 drops of Tween-20 (volume equivalent to 1% of the sodium hypochlorite solution) in the sodium hypochlorite solution, soak for 50 minutes, and rinse with sterile water after soaking Wash 6 times.
[0066] 5) Lay double-layer filter paper on the bottom of the watch glass, moisten it with sterile water, and be careful not to generate air bubbles....
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