Detection method for CVA10 in environmental water body

Abstract

The invention discloses a detection method for CVA10 in an environmental water body, wherein a semi-nested RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and real-time fluorescence quantification RT-PCR reaction system is established to detect CVA10 virus in the environmental water body by using a recombinant plasmid as a reference product, and the recombinant plasmid is prepared by using a primer and a probe aiming at CVA10 virus designed according to a genetic conservative fragment of enterovirus belonging to a VP1 serotype area. The method has good specificity and high sensitivity and precision, and can qualitatively and quantitatively detect CVA10 virus in the environmental water body accurately.

Description

technical field [0001] The invention belongs to the technical field of detection of environmental water quality, and in particular relates to a detection method of CVA10 virus in environmental water. technical background [0002] Coxsackievirus A10 (CVA10) is one of the main pathogens causing HFMD and belongs to the Enterovirus genus. CVA10 is a water-borne virus that can be released into environmental waters from the secretions of CVA10-infected patients and can survive in environmental waters for a long time. The water body polluted by it becomes an important pollution source of CVA10, which can cause the outbreak of hand, foot and mouth disease. [0003] Existing methods for detecting CVA10 mainly include cell culture method and polymerase chain reaction (Polymerase Chain Reaction, PCR). Because there are a variety of pathogenic microorganisms in the environmental water, especially other enteroviruses, these microorganisms interfere with the detection of CVA10 by cell c...

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Problems solved by technology

Because there are a variety of pathogenic microorganisms in the environmental water, especially other enteroviruses, these microorganisms interfere with the detection of CVA10 by cell culture, and it is difficult to distinguish and identify them by cell culture; PCR method is widely used in the detection of CVA10, but now Some PCR detection technologies are mainly used in the medical field. The detection samples basically come from human secretions. However, the concentration of CVA10 in environmental water is low, and the factors affecting the detection are complex. The existing PCR detection methods cannot accurately detect CVA10 in environmental water qualitatively or quantitatively.

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