Bacillus amyloliquefaciens probiotics capable of producing phytase and preparation as well as preparation method of probiotics

Abstract

The invention discloses bacillus amyloliquefaciens probiotics capable of producing phytase, and a preparation as well as a preparation method of the bacillus amyloliquefaciens probiotics. The gene GenBank accession number of the rRNA (Ribosomal Ribonucleic Acid) gene sequence of the bacillus amyloliquefaciens T6, 16S is JX081245. According to the method, a shallow tray fermentation culture method and a submerged fermentation culture method are applied to strain culture, and the specific steps include strain storage by glycerinum, strain activation, strain treatment in a shake flask, strain culture, load of the strain to a carrier, drying, inspection, and packaging to obtain a finished product. The novel probiotics preparation is prepared by using the bacillus amyloliquefaciens strain capable of producing the phytase, which is heat resistant, dryness resistant, acid resistant, alkali resistant and long in storage period, is stable in quality, and can be used for keeping intestinal microecological balance of livestock and poultry, ensuring that the growth and development of an animal in an ideal state reaches the maximum capacity of the animal, improving the feed digestion rate of the animal, improving the disease resistant capacity of the animal, promoting animal health, reducing the calcium phosphate addition amount in daily ration, reducing the excretion amount of the calcium phosphate in excrements of livestock and poultry, protecting environment and achieving the proposes of energy conservation and emission reduction.

Description

technical field [0001] The invention belongs to the field of preparation of probiotic preparations, and in particular relates to a probiotic of phytase-producing Bacillus amylolyticus, a preparation and a preparation method thereof. Background technique [0002] Probiotic preparations refer to active microbial cultures that can promote animal gastrointestinal microecological balance or enhance non-specific immune function to prevent diseases when ingested into animals, and indirectly promote animal growth and improve feed conversion rate. [0003] Studies have shown that probiotic preparations based on lactic acid bacteria and bacillus can replace antibiotic additives in feed. Feeding feed containing probiotic preparations can greatly reduce the amount of antibiotics used, avoid the problem of antibiotic residues in animals, and improve the quality of livestock and poultry products such as meat, eggs, and milk, which is of great significance to my country's breeding industry...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the embodiments of the present invention is to provide a phytase-producing Bacillus amylolyticus probiotic, preparation and preparation method thereof, aiming to solve the poor resistance of lactic acid bacteria probiotic preparations to adverse environmental factors such as drying, high temperature, acid-base, storage, etc. , there are not many types and quantities of lactic acid bacteria that can enter the intestinal tract alive, and other bacillus preparations do not produce phytase

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